Ohtomo T, Yoshida K
Department of Microbiology, St. Marianna University School of Medicine, Kawasaki, Japan.
Zentralbl Bakteriol Mikrobiol Hyg A. 1988 May;268(3):325-40. doi: 10.1016/s0176-6724(88)80017-8.
The adherence of an encapsulated strain of Staphylococcus aureus, S-P, and its variants to fibrinogen-, collagen-, and lectin-coated hydroxyapatite were compared. The parent strain, S-P, possesses a large capsule while its variants S-A and S-B possess a small capsule and microcapsule, respectively. The third variant, S-C, has no capsule. Adherence to proteinaceous substances varied according to the strains. While all four strains showed a similar degree of adhesion to collagen, the adhesion of strains S-A, S-B and S-C to fibrinogen and lectin differed from those of strain S-P. The effect of physical and enzymatic pretreatment of the strains on adhesion characteristics was measured. Generally, these results suggest that both carbohydrate and protein moieties on cell surface may be involved in adherence. In addition, the inhibition of adhesion by cell-surface polymers and monosaccharides was measured. The inhibition of adhesion of large capsulated (S-P) and unencapsulated (S-C) strains by proteinaceous substances differed. The large capsulated strain (S-P) of S. aureus had different adherence capacities in early-, mid-, or late log phases of growth, whereas the adherence capacities of the unencapsulated strain S-C remained nearly constant.
比较了一株包膜金黄色葡萄球菌S-P及其变体对纤维蛋白原、胶原蛋白和凝集素包被的羟基磷灰石的黏附情况。亲本菌株S-P具有大的荚膜，而其变体S-A和S-B分别具有小荚膜和微荚膜。第三个变体S-C没有荚膜。对蛋白质类物质的黏附因菌株而异。虽然所有四个菌株对胶原蛋白的黏附程度相似，但菌株S-A、S-B和S-C对纤维蛋白原和凝集素的黏附与菌株S-P不同。测定了菌株的物理和酶预处理对黏附特性的影响。总体而言，这些结果表明细胞表面的碳水化合物和蛋白质部分可能都参与了黏附。此外，还测定了细胞表面聚合物和单糖对黏附的抑制作用。蛋白质类物质对大荚膜（S-P）和无荚膜（S-C）菌株黏附的抑制作用不同。金黄色葡萄球菌的大荚膜菌株（S-P）在生长的早期、中期或晚期对数期具有不同的黏附能力，而无荚膜菌株S-C的黏附能力几乎保持恒定。
