Tuberculosis Prevention and Control Key Laboratory, Beijing Key Laboratory of New Techniques of Tuberculosis Diagnosis and Treatment, Tuberculosis Research Institute, the 8th Medical Center, Chinese PLA General Hospital, Beijing, 100091, China.
HeBei North University, Zhangjiakou, 075000, China.
Mil Med Res. 2021 Jun 2;8(1):34. doi: 10.1186/s40779-021-00324-5.
The traditional Chinese medicine NiuBeiXiaoHe (NBXH) extract and Chinese medicine preparation JieHeWan (JHW) exhibit anti-tuberculosis effects. The anti- tuberculosis effect of NBXH was compared with that of JHW to elucidate the mechanism of action of NBXH.
BALB/c mice aged 6-8 weeks were randomly divided into a normal control group, Tuberculosis (TB) model group, JHW treatment group, and NBXH treatment group. After 3 and 13 weeks of treatment, the therapeutic effect in each group was evaluated by comparing lung histopathology, lung and liver colony counts, the number of spots representing effector T cells secreting IFN-γ in an ELISPOT, and the levels of Th1, Th2, and Th17 cytokines, which were measured by a cytometric bead array (CBA). Mouse RNA samples were subjected to transcriptome sequencing.
After 13 weeks of treatment, the mean histopathological lesion area of the NBXH group was significantly smaller than that of the TB model group (P < 0.05). Compared with those in the TB model group, the lung colony counts in the JHW and NBXH groups were significantly decreased (P < 0.05), and the IL-2 and IL-4 levels in the NBXH group were significantly increased (P < 0.05). NBXH partly restored significant changes in gene expression caused by Mycobacterium tuberculosis (M. tuberculosis) infection. According to GO and KEGG analyses, the changes in biological process (BP), cell composition (CC) and molecular function (MF) terms and in signaling pathways caused by NBXH and JHW treatment were not completely consistent, but they were mainly related to the immune response and inflammatory response in the mouse TB model.
NBXH had therapeutic effects similar to those of JHW in improving lung histopathology, reducing lung colony counts, and regulating the levels of cytokines. NBXH restored significant changes in gene expression and repaired cell damage caused by M. tuberculosis infection by regulating immune-related pathways, which clarified the mechanism of action of NBXH.
中药牛贝晓赫（NBXH）提取物和中药制剂解热丸（JHW）具有抗结核作用。比较 NBXH 与 JHW 的抗结核作用，阐明 NBXH 的作用机制。
将 6-8 周龄 BALB/c 小鼠随机分为正常对照组、结核病（TB）模型组、JHW 治疗组和 NBXH 治疗组。治疗 3 周和 13 周后，通过比较各组肺组织病理学、肺和肝菌落计数、ELISPOT 中分泌 IFN-γ的效应 T 细胞斑点数以及 Th1、Th2 和 Th17 细胞因子水平，评价各组的治疗效果。细胞因子水平通过流式细胞术微珠阵列（CBA）进行测量。对小鼠 RNA 样本进行转录组测序。
治疗 13 周后，NBXH 组的平均组织病理学损伤面积明显小于 TB 模型组（P<0.05）。与 TB 模型组相比，JHW 和 NBXH 组的肺菌落计数明显降低（P<0.05），NBXH 组的 IL-2 和 IL-4 水平明显升高（P<0.05）。NBXH 部分恢复了结核分枝杆菌（M. tuberculosis）感染引起的基因表达的显著变化。根据 GO 和 KEGG 分析，NBXH 和 JHW 治疗引起的生物过程（BP）、细胞组成（CC）和分子功能（MF）术语以及信号通路的变化并不完全一致，但主要与小鼠 TB 模型中的免疫反应和炎症反应有关。
NBXH 与 JHW 相似，可改善肺组织病理学，降低肺菌落计数，调节细胞因子水平。NBXH 通过调节免疫相关途径，恢复结核分枝杆菌感染引起的基因表达的显著变化，修复细胞损伤，阐明了 NBXH 的作用机制。
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