Zhang Jianjun, Jiang Hongsheng, Feng Xuequan, Wang Liang, Yang Xinyu
Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin, China.
J BUON. 2021 Mar-Apr;26(2):388-394.
The purpose of this study was to investigate whether NBR2 can affect the proliferation of glioma cells by inhibiting the expression of p15, so as to promote the occurrence and development of glioma.
The expression of NBR2 in 44 glioma tissue specimens was detected by quantitative real-time polymerase chain reaction (qRT-PCR). The effects of NBR2 on cell viability, cell colony formation as well as cell migration and invasion abilities were examined by cell counting kit-8 (CCK-8) assay, plate cloning assay and Transwell assay. p15 protein was detected using Western blot. After simultaneous knockdown of NBR2 and p15, qRT-PCR, CCK-8, and plate cloning experiments were adopted to analyze p15 gene level, cell viability and proliferation ability, respectively.
NBR2 was highly expressed in glioma tissues, and the level in stage III/IV glioma tissues was conspicuously higher than that in stage I/II. The overall survival rate of glioma patients with high NBR2 level was conspicuously lower than those with low NBR2 expression. Clinical data analysis revealed that NBR2 expression was correlated with the WHO stage of clinical patients. After knockdown of NBR2, it was found that NBR2 level, cell viability, cell proliferation ability as well as migration and invasion abilities were all conspicuously reduced. In addition, the protein level of p15 was significantly increased after NBR2 was inhibited. Meanwhile, knockout of p15 could reverse the inhibitory effect of NBR2 on glioma cell proliferation.
The highly-expressed NBR2 inhibits the expression of p15, thus promoting the proliferation of glioma cells.
本研究旨在探讨NBR2是否可通过抑制p15的表达影响胶质瘤细胞增殖，进而促进胶质瘤的发生发展。
采用定量实时聚合酶链反应（qRT-PCR）检测44例胶质瘤组织标本中NBR2的表达。通过细胞计数试剂盒-8（CCK-8）法、平板克隆实验和Transwell实验检测NBR2对细胞活力、细胞集落形成以及细胞迁移和侵袭能力的影响。采用蛋白质免疫印迹法检测p15蛋白。同时敲低NBR2和p15后，分别采用qRT-PCR、CCK-8法和平板克隆实验分析p15基因水平、细胞活力和增殖能力。
NBR2在胶质瘤组织中高表达，Ⅲ/Ⅳ期胶质瘤组织中的水平明显高于Ⅰ/Ⅱ期。NBR2水平高的胶质瘤患者总生存率明显低于NBR2表达低的患者。临床数据分析显示，NBR2表达与临床患者的WHO分期相关。敲低NBR2后，发现NBR2水平、细胞活力、细胞增殖能力以及迁移和侵袭能力均明显降低。此外，抑制NBR2后p15蛋白水平显著升高。同时，敲除p15可逆转NBR2对胶质瘤细胞增殖的抑制作用。
高表达的NBR2抑制p15的表达，从而促进胶质瘤细胞的增殖。
