Department of Biotechnology, School of Engineering, Daegu University, Gyeongbuk, Republic of Korea.
Research Institute of Anti-Aging, Daegu University, Gyeongbuk, Republic of Korea.
Biofactors. 2021 Jul;47(4):658-664. doi: 10.1002/biof.1760. Epub 2021 Jun 2.
Cancerous inhibitor of protein phosphatase 2A (Cip2A) is an oncoprotein that promotes the development of several types of cancer. However, its molecular function in osteoblast differentiation remains unclear. In this study, we found that Cip2A was upregulated under osteogenic conditions in MG63 cells. Besides, overexpression of Cip2A significantly increased the expression of Runt-related transcription factor 2 (Runx2) and alkaline phosphatase (ALP). Inversely, the knockdown of Cip2A in MG63 cells suppressed osteoblast differentiation. Cip2A expression during osteogenic differentiation was mediated by extracellular signal-regulated kinase (ERK) activation. Taken together, our results suggest that Cip2A plays important role in regulating osteoblast differentiation by inducing ERK phosphorylation in MG63 cells.
癌蛋白磷酸酶 2A 的抑制剂（Cip2A）是一种致癌蛋白，可促进多种类型癌症的发展。然而，其在成骨细胞分化中的分子功能尚不清楚。在本研究中，我们发现 Cip2A 在 MG63 细胞的成骨条件下上调。此外，Cip2A 的过表达显著增加了 Runt 相关转录因子 2（Runx2）和碱性磷酸酶（ALP）的表达。相反，MG63 细胞中 Cip2A 的敲低抑制了成骨细胞分化。Cip2A 在成骨分化过程中的表达是通过细胞外信号调节激酶（ERK）的激活来介导的。综上所述，我们的结果表明 Cip2A 通过诱导 MG63 细胞中 ERK 的磷酸化在调节成骨细胞分化中发挥重要作用。
