Department of Veterinary Public Health, College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea.
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh.
Vector Borne Zoonotic Dis. 2021 Aug;21(8):579-585. doi: 10.1089/vbz.2020.2738. Epub 2021 Jun 2.
The immune response to mainly depends on antigen-specific T cell activation, CD4 and CD8 T cells, and specific humoral response. Protective immune response against infection has not been performed in the Sprague-Dawley (SD) rat model. We measured bacterial kinetics in addition to and interferon gamma (IFN-γ) and interleukin-10 (IL-10) production against crude protein in the SD rats at different days of postinfection with biotype 1 by indirect enzyme-linked immunosorbent assay. Forty SD rats were inoculated intraperitoneally with 0.1 mL sterile injectable pyrogen-free solution containing 1 × 10 colony-forming units/mL of biotype 1 obtained from cattle in Korea. Four rats were used as uninfected control. Serum IFN-γ level at 3 and 7 days postinfection were significantly higher ( > 0.001) compared with the IL-10 level. On the contrary, serum IL-10 levels were observed significantly higher at 21 and 28 days postinfection compared with the serum IFN-γ levels ( < 0.001). The production of IFN-γ by spleen cells was significantly higher at 7 and 14 days postinfection compared with IL-10 ( < 0.001). On the contrary, IL-10 productions were found to be significantly higher at 21, 28, 35, and 42 days postinfection compared with IFN-γ ( < 0.001). The presence of in blood was marked till 5 weeks of infection, throughout the experiment in case of spleen, and no bacteria were isolated from the kidney and liver at 6 weeks postinfection. The and IFN-γ and IL-10 measurement in our study reported that infection in rats primarily educe T helper (Th)1-dominant immune response in acute infection accompanied by Th2-dominant immune response in chronic infection.
针对 感染的免疫反应主要依赖于抗原特异性 T 细胞激活、CD4 和 CD8 T 细胞以及特异性体液反应。在 SD 大鼠模型中，尚未针对 感染产生保护性免疫反应。我们通过间接酶联免疫吸附试验，在感染 生物型 1 后不同天数测量了 SD 大鼠中的细菌动力学以及针对粗 蛋白的 干扰素γ (IFN-γ) 和白细胞介素-10 (IL-10) 的产生。40 只 SD 大鼠经腹腔接种 0.1 mL 无菌无热原注射溶液，其中含有 1 × 10 个 CFU/mL 来自韩国牛的 生物型 1。4 只大鼠作为未感染对照。感染后 3 天和 7 天血清 IFN-γ 水平明显高于 IL-10 水平（ > 0.001）。相反，感染后 21 天和 28 天血清 IL-10 水平明显高于 IFN-γ 水平（ < 0.001）。感染后 7 天和 14 天脾细胞产生的 IFN-γ明显高于 IL-10（ < 0.001）。相反，在感染后 21、28、35 和 42 天，IL-10 的产生明显高于 IFN-γ（ < 0.001）。在整个实验过程中，感染后 5 周内血液中均存在 ，脾脏中存在 ，而在感染后 6 周时，肾脏和肝脏中均未分离出细菌。我们的研究中对 及 IFN-γ 和 IL-10 的测量表明，大鼠 感染主要在急性感染时诱导 Th1 优势免疫反应，在慢性感染时诱导 Th2 优势免疫反应。
