用抗体包被的金胶体检测生物素化核酸杂交体。

Detection of biotinylated nucleic acid hybrids by antibody-coated gold colloid.

作者信息

Tomlinson S, Lyga A, Huguenel E, Dattagupta N

机构信息

Molecular Diagnostics Inc., Miles Research Center, West Haven, Connecticut 06516.

出版信息

Anal Biochem. 1988 May 15;171(1):217-22. doi: 10.1016/0003-2697(88)90145-5.

DOI:10.1016/0003-2697(88)90145-5

PMID:3407918

Abstract

A simple and sensitive nonradioactive method for detecting biotinylated nucleic acid hybrids on nitrocellulose paper is described. The method employs anti-biotin antibody-coated gold colloid and silver enhancement. Comparative results indicate sensitivity similar to that of a commonly used enzymatic colorimetric detection method.

摘要

本文描述了一种用于检测硝酸纤维素纸上生物素化核酸杂交体的简单且灵敏的非放射性方法。该方法采用抗生物素抗体包被的金胶体和银增强技术。对比结果表明，其灵敏度与常用的酶比色检测方法相似。