矿物油可增强对结合在硝酸纤维素膜上的32P标记核酸的放射自显影检测。

Mineral oil enhances the autoradiographic detection of 32P-labeled nucleic acids bound to nitrocellulose membranes.

作者信息

Rust S, Funke H, Assmann G

出版信息

Anal Biochem. 1987 May 15;163(1):196-9. doi: 10.1016/0003-2697(87)90112-6.

PMID:3619019

Abstract

The autoradiographic signal obtained from 32P-labeled nucleic acids hybridized to nitrocellulose membrane-bound DNA or RNA can be enhanced by making the membrane translucent with mineral oil and placing it between two intensifying screens. Elution of the mineral oil after autoradiography allows the reuse of the membranes in subsequent hybridizations.

摘要

通过用矿物油使硝酸纤维素膜结合的DNA或RNA杂交得到的32P标记核酸的放射自显影信号，可以通过用矿物油使膜半透明并将其置于两个增感屏之间来增强。放射自显影后洗脱矿物油可使膜在后续杂交中重复使用。