Department of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, Canada.
Department of Small Animal Clinical Sciences, University of Saskatchewan, Saskatoon, Canada.
Ann Anat. 2022 Jan;239:151774. doi: 10.1016/j.aanat.2021.151774. Epub 2021 May 31.
Nucleobindin2 (NUCB2)/nesfatin-1 expression in human plasma positively correlates with the expression of pro-inflammatory cytokines in patients with chronic obstructive pulmonary disease (COPD), implicating its potential role in neutrophilic lung inflammation. There are no data on the localization of nucleobindin2 (NUCB2)/nesfatin-1 in human lungs and inflammatory cells. We examined the localization of NUCB2/nesfatin-1-immunoreactivity in normal and inflamed human lungs obtained from COPD patients and neutrophils with light and immunoelectron microscopy. Immunohistology showed localization of NUCB2/nesfatin-1-like immunoreactivity in the bronchiolar epithelium, alveolar septa, vascular endothelium and various immune cells of normal and inflamed lungs. Further, NUCB2/nesfatin-1-like immunoreactivity accumulated within 0.5 μm of the plasma membrane in human neutrophils following 90 min of 1 ng/mL LPS stimulation. NUCB2/nesfatin-1-like immunoreactivity was also found to localize in euchromatic portions of neutrophilic nuclei at five times the mean concentration compared to heterochromatin. Finally, our results indicate that NUCB2/nesfatin-1-like immunoreactivity is predominantly cytoplasmic including that in the Golgi complex and vesicles as it localizes at two times the concentration in neutrophilic cytoplasm compared to nucleus. Our study is the first to detail the localization of NUCB2/nesfatin-1-like immunoreactivity in lungs and neutrophils, and nuclear localization of NUCB2/nesfatin-1 also implicates its potential role in transcriptional regulation.
核结合蛋白 2（NUCB2）/nesfatin-1 在人类血浆中的表达与慢性阻塞性肺疾病（COPD）患者促炎细胞因子的表达呈正相关，提示其在中性粒细胞性肺炎症中可能具有潜在作用。目前尚无关于核结合蛋白 2（NUCB2）/nesfatin-1 在人肺和炎症细胞中定位的研究数据。我们通过光镜和免疫电镜检查了 COPD 患者正常和炎症肺组织以及中性粒细胞中 NUCB2/nesfatin-1 免疫反应性的定位。免疫组织化学显示，NUCB2/nesfatin-1 样免疫反应性定位于正常和炎症肺的细支气管上皮、肺泡隔、血管内皮和各种免疫细胞中。此外，在 1ng/ml LPS 刺激 90 分钟后，人类中性粒细胞中 NUCB2/nesfatin-1 样免疫反应性在质膜 0.5μm 内聚集。与异染色质相比，NUCB2/nesfatin-1 样免疫反应性在中性粒细胞的常染色质部分的浓度也增加了五倍。最后，我们的结果表明，NUCB2/nesfatin-1 样免疫反应性主要定位于细胞质中，包括高尔基体复合体和小泡，因为与核相比，中性粒细胞细胞质中的浓度是核的两倍。本研究首次详细描述了 NUCB2/nesfatin-1 样免疫反应性在肺和中性粒细胞中的定位，以及核内定位的 NUCB2/nesfatin-1 也暗示其在转录调节中的潜在作用。
