比较两种 β-D-葡聚糖检测方法在免疫功能低下患者侵袭性真菌感染中的应用。

Comparison of two β-D-glucan assays for detecting invasive fungal diseases in immunocompromised patients.

机构信息

Department of Laboratory Medicine, Yonsei University College of Medicine, Seodaemun-gu, Seoul, Korea.

出版信息

Diagn Microbiol Infect Dis. 2021 Sep;101(1):115415. doi: 10.1016/j.diagmicrobio.2021.115415. Epub 2021 May 5.

DOI:10.1016/j.diagmicrobio.2021.115415

Abstract

(1-3)-β-D-glucan (BDG) is a major biomarker of invasive fungal diseases (IFDs), which are life-threatening for immunodeficient patients. We compared the clinical performance of two BDG-detection assays. The precision, linearity, reference interval, and limit of quantitation of the Wako BDG assay were analyzed and the performance was compared with that of the Goldstream BDG assay using 272 clinical serum samples. The repeatability, within-laboratory imprecision, and limit of quantitation of the Wako BDG assay were 3.8%, 5.9%, and 7.35 pg/mL, respectively (linearity, 23.8-557 pg/mL; R = 0.998). The correlation coefficient, slope, and y-intercept for the Wako BDG assay versus Goldstream BDG assay were 0.29, 3.82, and 0.04, respectively. The sensitivity and specificity were 43.8% and 94.9% for the Wako BDG assay and 39.6% and 83.5% for the Goldstream BDG assay, respectively. In clinical settings, the Wako BDG assay is suitable for diagnosing patients with IFDs.

摘要

(1-3)-β-D-葡聚糖（BDG）是侵袭性真菌病（IFD）的主要生物标志物，对免疫功能低下的患者有生命威胁。我们比较了两种 BDG 检测方法的临床性能。分析了和光 BDG 检测法的精密度、线性、参考区间和定量下限，并使用 272 份临床血清样本比较了其与 Goldstream BDG 检测法的性能。和光 BDG 检测法的重复性、实验室内部不精密度和定量下限分别为 3.8%、5.9%和 7.35pg/mL（线性范围为 23.8-557pg/mL；R=0.998）。和光 BDG 检测法与 Goldstream BDG 检测法的相关系数、斜率和 y 截距分别为 0.29、3.82 和 0.04。和光 BDG 检测法的灵敏度和特异性分别为 43.8%和 94.9%，Goldstream BDG 检测法分别为 39.6%和 83.5%。在临床环境中，和光 BDG 检测法适用于诊断 IFD 患者。