College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, China.
College of Materials Science and Engineering, Taiyuan University of Technology, Taiyuan 030024, China.
Mater Sci Eng C Mater Biol Appl. 2021 Jul;126:112118. doi: 10.1016/j.msec.2021.112118. Epub 2021 Apr 21.
Fibrous materials are of great interest in the development of tissue regenerative matrix. However, synthesis of inorganic fibrous microspheres as cell carriers is a great challenge. In this study, we for the first time report on the synthesis of novel hierarchical and flower-like TiO nanowire (NW) microspheres as biocompatible cell carriers. TiO NW microspheres were synthesized through in situ alkali hydrothermal treatment of the TiO nanoparticle (NP) microspheres and their microstructure, formation mechanism and in vitro biocompatibility were evaluated. SEM observations show that the resulting TiO NW microspheres were constructed by a large number of NWs with the diameter of 10-20 nm and exhibited a flower-like and hierarchical morphology with the diameter of 400-600 μm. XRD patterns indicate that TiO NW microspheres were constructed by both rutile and anatase phase of TiO. FT-IR spectra reveal that Ti-O-Ti bonds were involved in TiO NW microspheres. In vitro biocompatibility was evaluated by seeding the fibroblast L929 cells on the microspheres. A conventional MTT assay quantitatively indicates that the TiO NW microspheres favored adhesion and proliferation of cells and were biocompatible, while SEM observations qualitatively confirmed that the cells were well grown on the surface of TiO NW microspheres. Thus, the as-synthesized TiO NW microspheres would be applicable to novel and biocompatible cell carriers.
纤维材料在组织再生基质的开发中具有重要意义。然而，作为细胞载体的无机纤维微球的合成是一个巨大的挑战。在本研究中，我们首次报道了新型分级花状 TiO 纳米线（NW）微球作为生物相容性细胞载体的合成。通过 TiO 纳米颗粒（NP）微球的原位碱水热处理合成了 TiO NW 微球，并对其微观结构、形成机制和体外生物相容性进行了评价。SEM 观察表明，所得的 TiO NW 微球由大量直径为 10-20nm 的 NW 组成，并具有 400-600μm 的花状和分级形貌。XRD 图谱表明，TiO NW 微球由 TiO 的金红石相和锐钛矿相组成。FT-IR 谱表明，Ti-O-Ti 键参与了 TiO NW 微球的形成。通过将成纤维细胞 L929 细胞接种在微球上评估体外生物相容性。传统的 MTT 分析定量表明，TiO NW 微球有利于细胞的黏附和增殖，具有生物相容性，而 SEM 观察定性证实细胞在 TiO NW 微球表面生长良好。因此，所合成的 TiO NW 微球可适用于新型生物相容性细胞载体。
