Wu K S, Glaszmann J C, Khush G S
Department of Agronomy, South China Agricultural University, Guangzhou.
Biochem Genet. 1988 Apr;26(3-4):303-20. doi: 10.1007/BF00561468.
Chromosomal locations of 10 isozyme loci in rice (Oryza sativa L.) were determined through trisomic analysis. All 10 genes produced altered allozyme banding patterns in specific F1 trisomics. This served as the primary source of evidence for chromosome locations of Est-5, Icd-1, Acp-1, and Pgd-1. The locations of Amp-1, Amp-2, Amp-4, Pox-5, Got-1, and Cat-1 were further confirmed from segregation data in BC1 generations, as the ratios deviated significantly from 1:1 in the critical trisomics but agreed with the expected trisomic ratios. Triallelic heterozygotes were recovered for Amp-1 and Amp-2. On the basis of these data Got-1, Est-5, and Icd-1 were located to chromosome 1, Amp-1 to chromosome 2, Cat-1 and Pox-5 to chromosome 3, Acp-1 to chromosome 6, Amp-2 and Amp-4 to chromosome 8, and Pgd-1 to chromosome 11. Because Acp-2 and Pox-2 are known to be linked with Acp-1, they must also be on chromosome 6. The gene order and recombination values between isozyme loci on chromosomes 3, 6, 8, and 11 are presented.
通过三体分析确定了水稻（Oryza sativa L.）中10个同工酶基因座的染色体位置。所有10个基因在特定的F1三体中产生了改变的等位酶带型。这是Est-5、Icd-1、Acp-1和Pgd-1染色体定位的主要证据来源。Amp-1、Amp-2、Amp-4、Pox-5、Got-1和Cat-1的位置从BC1代的分离数据中得到进一步证实，因为在关键三体中其比例显著偏离1:1，但与预期的三体比例一致。获得了Amp-1和Amp-2的三基因杂合子。基于这些数据，Got-1、Est-5和Icd-1定位到第1染色体，Amp-1定位到第2染色体，Cat-1和Pox-5定位到第3染色体，Acp-1定位到第6染色体，Amp-2和Amp-4定位到第8染色体，Pgd-1定位到第11染色体。由于已知Acp-2和Pox-2与Acp-1连锁，它们也必定在第6染色体上。给出了第3、6、8和11染色体上同工酶基因座之间的基因顺序和重组值。
