牛tRNA修饰基因的遗传变异检测及启动子区域活性分析

Detection of genetic variation and activity analysis of the promoter region of the cattle tRNA-modified gene .

作者信息

Yi Xiaohua, He Shuai, Wang Shuhui, Zhao Haidong, Wu Mingli, Liu Shirong, Sun Xiuzhu

机构信息

College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, PR China.

College of Grassland Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, PR China.

出版信息

Arch Anim Breed. 2021 Apr 30;64(1):147-155. doi: 10.5194/aab-64-147-2021. eCollection 2021.

DOI:10.5194/aab-64-147-2021

PMID:34084913

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8162237/

Abstract

The tRNA modification gene in eukaryotes is relatively conservative. As an important modification gene, the gene plays an important role in maintaining tRNA structural maintenance and reducing mistranslation of protein translation by methylation of specific tRNA subpopulations. Mouse and zebrafish knockout experiments indicate that it may mediate growth and development through tRNA modification. However, there are no systematic reports on the function of tRNA-modified genes in livestock. In this study, Qinchuan cattle DNA pool sequencing technology was used. A G C mutation in the  bp position upstream of the translation initiator codon was found. At this locus, the dual-luciferase assay indicated that different genotypes cause differences in transcriptional activity ( ). Our experiment detected a natural genetic variation of a tRNA modification gene , which may provide potential natural molecular materials for the study of tRNA modification.

摘要

真核生物中的tRNA修饰基因相对保守。作为一个重要的修饰基因，该基因在维持tRNA结构以及通过特定tRNA亚群的甲基化减少蛋白质翻译的错误翻译方面发挥着重要作用。小鼠和斑马鱼的基因敲除实验表明，它可能通过tRNA修饰介导生长和发育。然而，关于家畜中tRNA修饰基因功能尚无系统报道。本研究利用秦川牛DNA池测序技术，在翻译起始密码子上游 bp位置发现了一个G→C突变。在此位点，双荧光素酶检测表明不同基因型导致转录活性存在差异（）。我们的实验检测到一个tRNA修饰基因的自然遗传变异，这可能为tRNA修饰研究提供潜在的天然分子材料。