Liu Xuan, Usman Tahir, Wang Yachun, Wang Zezhao, Xu Xianzhou, Wu Meng, Zhang Yi, Zhang Xu, Li Qiang, Liu Lin, Shi Wanhai, Qin Chunhua, Geng Fanjun, Wang Congyong, Tan Rui, Huang Xixia, Liu Airong, Wu Hongjun, Tan Shixin, Yu Ying
Key Laboratory of Agricultural Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Key Laboratory of Agricultural Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China ; Department of Animal Health, The University of Agriculture, Peshawar, Peshawar 25000, Pakistan .
Asian-Australas J Anim Sci. 2015 Apr;28(4):467-75. doi: 10.5713/ajas.13.0837.
Improvement for carcass traits related to beef quality is the key concern in beef production. Recent reports found that epigenetics mediates the interaction of individuals with environment and nutrition. The present study was designed to analyze the genetic effect of single nucleotide polymorphisms (SNPs) in seven epigenetic-related genes (DNMT1, DNMT3a, DNMT3b, DNMT3L, Ago1, Ago2, and HDAC5) and two meat quality candidate genes (CAPN1 and PRKAG3) on fourteen carcass traits related to beef quality in a Snow Dragon beef population, and also to identify SNPs in a total of fourteen cattle populations. Sixteen SNPs were identified and genotyped in 383 individuals sampled from the 14 cattle breeds, which included 147 samples from the Snow Dragon beef population. Data analysis showed significant association of 8 SNPs within 4 genes related to carcass and/or meat quality traits in the beef populations. SNP1 (13154420A>G) in exon 17 of DNMT1 was significantly associated with rib-eye width and lean meat color score (p<0.05). A novel SNP (SNP4, 76198537A>G) of DNMT3a was significantly associated with six beef quality traits. Those individuals with the wild-type genotype AA of DNMT3a showed an increase in carcass weight, chilled carcass weight, flank thicknesses, chuck short rib thickness, chuck short rib score and in chuck flap weight in contrast to the GG genotype. Five out of six SNPs in DNMT3b gene were significantly associated with three beef quality traits. SNP15 (45219258C>T) in CAPN1 was significantly associated with chuck short rib thickness and lean meat color score (p<0.05). The significant effect of SNP15 on lean meat color score individually and in combination with each of other 14 SNPs qualify this SNP to be used as potential marker for improving the trait. In addition, the frequencies of most wild-type alleles were higher than those of the mutant alleles in the native and foreign cattle breeds. Seven SNPs were identified in the epigenetic-related genes. The SNP15 in CAPN1 could be used as a powerful genetic marker in selection programs for beef quality improvement in the Snow Dragon Beef population.
提高与牛肉品质相关的胴体性状是牛肉生产中的关键问题。最近的报告发现，表观遗传学介导个体与环境和营养之间的相互作用。本研究旨在分析七个表观遗传相关基因（DNMT1、DNMT3a、DNMT3b、DNMT3L、Ago1、Ago2和HDAC5）和两个肉质候选基因（CAPN1和PRKAG3）中的单核苷酸多态性（SNP）对雪龙牛肉群体中与牛肉品质相关的14个胴体性状的遗传效应，并在总共14个牛群体中鉴定SNP。在从14个牛品种中采集的383个个体中鉴定出16个SNP并进行基因分型，其中包括来自雪龙牛肉群体的147个样本。数据分析表明，牛肉群体中4个与胴体和/或肉质性状相关的基因内的8个SNP存在显著关联。DNMT1第17外显子中的SNP1（13154420A>G）与肋眼宽度和瘦肉颜色评分显著相关（p<0.05）。DNMT3a的一个新SNP（SNP4，76198537A>G）与六个牛肉品质性状显著相关。与GG基因型相比，DNMT3a野生型基因型AA的个体在胴体重、冷却胴体重、侧腹厚度、肩胛短肋厚度、肩胛短肋评分和肩胛肉瓣重量方面有所增加。DNMT3b基因的六个SNP中有五个与三个牛肉品质性状显著相关。CAPN1中的SNP15（45219258C>T）与肩胛短肋厚度和瘦肉颜色评分显著相关（p<0.05）。SNP15对瘦肉颜色评分的显著效应单独以及与其他14个SNP中的每一个组合，使该SNP有资格用作改善该性状的潜在标记。此外，在本地和外国牛品种中，大多数野生型等位基因的频率高于突变等位基因。在表观遗传相关基因中鉴定出7个SNP。CAPN1中的SNP15可作为雪龙牛肉群体中改善牛肉品质选择计划的有力遗传标记。
