Li Na, Hong Su-Yun, Li Jun, Wang Gai-Mei, Liu Zhe, Liang Yi
Rehabilitation of Acupuncture-Moxibustion and Tuina, Ningbo Municipal Fenghua District Hospital of TCM, Ningbo 315500, Zhejiang Province, China; Laboratory Room of Neurobiology and Acupuncture Research, Third Clinical Medical College of Zhejiang Chinese Medical University, Hangzhou 310053.
Laboratory Room of Neurobiology and Acupuncture Research, Third Clinical Medical College of Zhejiang Chinese Medical University, Hangzhou 310053.
Zhongguo Zhen Jiu. 2021 Jun 12;41(6):645-50. doi: 10.13703/j.0255-2930.20200415-0008.
To observe the effect of electroacupuncture (EA) pretreatment on hippocampal oxidative stress in aged mice with postoperative cognitive dysfunction (POCD) and explore the relevant mechanism of EA pretreatment on the improvement of learning and memory in POCD aged mice.
A total of 72 healthy male aged mice were randomized into a blank group, a model group, a medication group and an EA group, 18 mice in each one. In each group, 1-day, 3-day and 7-day subgroups were divided separately, 6 mice in each subgroup. In the EA group, "Baihui" (GV 20) and "Dazhui" (GV 14) were selected and stimulated with EA, using continuous wave (15 Hz, 1 mA), continuously for 30 min, once a day, for 5 days consecutively. In the medication group, 10% minocycline was injected intraperitoneally, 40 mg/kg, once a day, consecutively for 5 days. In the blank and the control group, intraperitoneal injection of 0.9% sodium chloride solution was given with equal dosage. Except the blank group, at the end of intervention, partial hepatectomy was conducted to establish POCD model in the rest groups. Morris water maze test was adopted to evaluate the learning and memory ability of the aged mice. ELISA was used to determine the contents of reactive oxygen species (ROS) and malondialdehyde (MDA) in the hippocampal tissue. Western blot method was applied to detect the protein expressions of superoxide dismutase 1 (SOD 1) and superoxide dismutase 2 (SOD 2) in the hippocampal tissue.
Compared with the blank group, the percentage of platform quadrant residence time was obviously reduced in the mice in the model group (<0.01), and those in the medication group and the EA group were larger than the model group (<0.01). Compared with the model group, the contents of ROS were reduced in each subgroup of the medication group and the 3-day subgroup and the 7-day subgroup of the EA group separately (<0.01, <0.05), MDA contents were reduced in the medication group and the EA group (<0.01, <0.05), the protein expressions of SOD 1 and SOD 2 were all increased in each subgroup of the medication group and the 3-day subgroup and the 7-day subgroup of the EA group (<0.05, <0.01). Compared with the medication group, the contents of ROS and MDA in the 1-day subgroup of the EA group were increased (<0.05), the hippocampal SOD 1 protein expression was increased in the 7-day group of the EA group (<0.05) and the hippocampal SOD 2 protein expression was reduced in the 1-day subgroup of the EA group (<0.05).
Electroacupuncture pretreatment at "Baihui" (GV 20) and "Dazhui" (GV 14) may increase the learning and memory ability of POCD aged mice, which is probably related to the decrease of oxidative stress and the strengthening of hippocampal antioxidant capacity.
观察电针预处理对老年术后认知功能障碍（POCD）小鼠海马氧化应激的影响，探讨电针预处理改善POCD老年小鼠学习记忆的相关机制。
将72只健康雄性老年小鼠随机分为空白组、模型组、药物组和电针组，每组18只。每组再分别分为1天、3天和7天亚组，每个亚组6只。电针组选取“百会”（GV 20）和“大椎”（GV 14）进行电针刺激，采用连续波（15Hz，1mA），持续30分钟，每天1次，连续5天。药物组腹腔注射10%米诺环素，40mg/kg，每天1次，连续5天。空白组和对照组腹腔注射等剂量的0.9%氯化钠溶液。除空白组外，干预结束时，其余组行部分肝切除术建立POCD模型。采用Morris水迷宫试验评估老年小鼠的学习记忆能力。采用ELISA法测定海马组织中活性氧（ROS）和丙二醛（MDA）含量。采用Western blot法检测海马组织中超氧化物歧化酶1（SOD 1）和超氧化物歧化酶2（SOD 2）的蛋白表达。
与空白组比较，模型组小鼠平台象限停留时间百分比明显降低（<0.01），药物组和电针组小鼠平台象限停留时间百分比均高于模型组（<0.01）。与模型组比较，药物组各亚组及电针组3天亚组和7天亚组的ROS含量均降低（<0.01，<0.05），药物组和电针组MDA含量降低（<0.01，<0.05），药物组各亚组及电针组3天亚组和7天亚组的SOD 1和SOD 2蛋白表达均升高（<0.05，<0.01）。与药物组比较，电针组1天亚组的ROS和MDA含量升高（<0.05），电针组7天亚组海马SOD 1蛋白表达升高（<0.05），电针组1天亚组海马SOD 2蛋白表达降低（<0.05）。
“百会”（GV 20）和“大椎”（GV 14）电针预处理可能提高POCD老年小鼠的学习记忆能力，其机制可能与减轻氧化应激、增强海马抗氧化能力有关。
