固定在白蛋白微球上的蛋白质的功能特性。
Functional properties of proteins immobilized on albumin microspheres.
作者信息
Yan C H, Kitano H, Ise N
机构信息
Department of Polymer Chemistry, Kyoto University, Japan.
出版信息
Biotechnol Appl Biochem. 1988 Feb;10(1):13-20.
Bovine serum albumin (BSA) microspheres with an average diameter of 12.5 micron were prepared by crosslinking of BSA molecules with glutaraldehyde in the presence of polymethylmethacrylate dissolved in chloroform-toluene. Trypsin and anti-human IgG antibody were immobilized onto their surfaces by the glutaraldehyde-activation method. The catalytic activity and storage stability of the immobilized trypsin were satisfactorily high. The enzyme immunoassay (EIA) method using BSA-microspheres as a solid phase has a high sensitivity (the minimum concentration of detectable antigen in the sample: 0.2 ng/ml) and a wide concentration range (final concentration 0.027-3000 ng/ml) for the detection of human IgG.
通过在溶解于氯仿 - 甲苯的聚甲基丙烯酸甲酯存在下，使牛血清白蛋白（BSA）分子与戊二醛交联，制备了平均直径为12.5微米的BSA微球。通过戊二醛活化法将胰蛋白酶和抗人IgG抗体固定在其表面。固定化胰蛋白酶的催化活性和储存稳定性令人满意地高。使用BSA微球作为固相的酶免疫测定（EIA）方法对于检测人IgG具有高灵敏度（样品中可检测抗原的最低浓度：0.2 ng/ml）和宽浓度范围（终浓度0.027 - 3000 ng/ml）。
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