Department of Bariatric Surgery, The First Affiliated Hospital of Jinan University, Guangzhou, China.
Feinberg School of Medicine, Robert H Lurie Comprehensive Cancer Center, Northwestern University, Chicago, IL, USA.
Adipocyte. 2021 Dec;10(1):310-321. doi: 10.1080/21623945.2021.1933297.
Adipocytes and adipocyte lipid metabolism are closely related with obesity and type 2 diabetes, but the molecular mechanism still needs further investigation. The aim of this study is to discover the adipocyte genes and pathways involved in obesity and type 2 diabetes using bioinformatics analysis. The GSE27951 gene expression profile was obtained. Software and online tools (STRING, Cytoscape, BioGPS, CTD, and FunRich) were used to identify core genes.21 human subcutaneous adipose samples, with 10 from type 2 diabetic patients and 11 from normal controls, were included in these analyses. 184 differentially expressed genes (DEGs) including 42 up-regulated genes and 142 down-regulated genes were found to be enriched in metabolism, receptor activity, collagen type IV and glutamine biosynthesis I pathway by using the enrichment analysis. Seven hub genes were identified from the PPI network using various software (Cytoscape, STRING, BioGPS, and CTD). Four core genes (COL4A2, ACACB, GLUL, and CD36) were found to be highly expressed in subcutaneous adipose tissue of obese patients accompanying type 2 diabetes. COL4A2, ACACB, GLUL and CD36 might be the core molecular biomarkers of obesity in patients with or without type 2 diabetes.
脂肪细胞和脂肪细胞脂质代谢与肥胖和 2 型糖尿病密切相关，但分子机制仍需进一步研究。本研究旨在通过生物信息学分析发现与肥胖和 2 型糖尿病相关的脂肪细胞基因和途径。获得了 GSE27951 基因表达谱。使用软件和在线工具（STRING、Cytoscape、BioGPS、CTD 和 FunRich）来识别核心基因。对 21 个人体皮下脂肪样本进行了分析，其中 10 例来自 2 型糖尿病患者，11 例来自正常对照者。通过富集分析发现，有 184 个差异表达基因（DEGs），包括 42 个上调基因和 142 个下调基因，富集在代谢、受体活性、IV 型胶原和谷氨酰胺生物合成 I 途径中。使用各种软件（Cytoscape、STRING、BioGPS 和 CTD）从 PPI 网络中鉴定出 7 个枢纽基因。在肥胖合并 2 型糖尿病患者的皮下脂肪组织中发现 4 个核心基因（COL4A2、ACACB、GLUL 和 CD36）表达较高。COL4A2、ACACB、GLUL 和 CD36 可能是肥胖患者（无论是否患有 2 型糖尿病）的核心分子生物标志物。
