Department of Obstetrics & Gynecology, The University of Texas Medical Branch at Galveston, 301 University Boulevard, Galveston, TX, 77555, USA.
AdvaGenix, 9430 Key West Ave. Suite 130, Rockville, MD, 20850, USA.
J Assist Reprod Genet. 2021 Sep;38(9):2363-2370. doi: 10.1007/s10815-021-02250-w. Epub 2021 Jun 4.
The goal is to determine if variations exist between male and female blastocysts in preimplantation measurements of quality and ploidy and in vitro fertilization elective single-embryo transfer (eSET) outcomes.
A retrospective chart review was conducted from a private fertility center's database of blastocysts undergoing preimplantation genetic testing for aneuploidy, along with details of eSET from this screened cohort. Main outcomes included preimplantation embryo quality and sex-specific eSET outcomes.
A total of 3708 embryos from 578 women were evaluated, with 45.9% male and 54.1% female. The majority were High grade. No difference existed between embryo sex and overall morphological grade, inner cell mass or trophectoderm grade, or blastocyst transformation day. Female blastocysts had a higher aneuploidy rate than male blastocysts (P < 0.001). Five hundred thirty-nine eSETs from 392 women were evaluated, with High grade embryos more likely to have implantation (P < 0.001), clinical pregnancy (P < 0.001), and ongoing pregnancy (P = 0.018) than Mid or Low grade embryos. Day 5 blastocysts were more likely to have implantation (P = 0.018), clinical pregnancy (P = 0.005), and ongoing pregnancy (P = 0.018) than day 6 blastocysts. Male and female embryos had similar transfer outcomes, although female day 5 blastocysts were more likely to result in clinical pregnancy (P = 0.012), but not ongoing pregnancy, than female day 6 blastocysts. Male eSET outcomes did not differ by blastocyst transformation day.
Male and female embryos have comparable grade and quality; however, female embryos were more likely to be aneuploid. Ongoing pregnancy rates did not differ by embryo sex. Day 5 embryos had more favorable transfer outcomes than day 6 embryos.
本研究旨在探讨在胚胎植入前遗传学检测（PGT）中，雄性和雌性囊胚的质量和倍性是否存在差异，以及体外受精选择性单胚胎移植（eSET）的结局是否存在差异。
回顾性分析了一家私人生育中心的囊胚 PGT 数据库，这些囊胚来自于筛选队列的 eSET 详细信息。主要结局包括胚胎植入前的胚胎质量和性别特异性的 eSET 结局。
共评估了 578 名女性的 3708 个胚胎，其中 45.9%为男性，54.1%为女性。大多数胚胎为高等级。胚胎性别与整体形态学等级、内细胞团或滋养外胚层等级或囊胚孵育日无差异。女性囊胚的非整倍体率高于男性囊胚（P<0.001）。共评估了 392 名女性的 539 个 eSET，高等级胚胎更有可能发生种植（P<0.001）、临床妊娠（P<0.001）和持续妊娠（P=0.018），而中等级或低等级胚胎则不然。第 5 天的囊胚更有可能发生种植（P=0.018）、临床妊娠（P=0.005）和持续妊娠（P=0.018），而第 6 天的囊胚则不然。虽然女性第 5 天的囊胚更有可能导致临床妊娠（P=0.012），但不是持续妊娠，而女性第 6 天的囊胚更有可能发生，但男性和女性胚胎的移植结局相似。囊胚孵育日对男性胚胎的移植结局没有影响。
雄性和雌性胚胎具有相似的等级和质量；然而，雌性胚胎更有可能是非整倍体。妊娠结局不受胚胎性别的影响。第 5 天的胚胎的移植结局比第 6 天的胚胎更有利。
