HIV合并感染对HTLV-1感染患者免疫生物标志物谱的影响。

Impact of HIV co-infection on immunological biomarker profile of HTLV-1 infected patients.

作者信息

Starling Ana Lúcia Borges, Pereira Sílvio Roberto Souza, Peruhype-Magalhães Vanessa, Coelho-Dos-Reis Jordana Grazziela Alves, Bicalho Kelly Alves, de Paiva Luciene Pimenta, Martins Julia Pereira, Trindade Bruno Caetano, Labanca Ludimila, Faccioli Lúcia Helena, Lambertucci José Roberto, Silva Luciana Cristina Dos Santos, Antunes Carlos Maurício de Figueiredo, Teixeira-Carvalho Andréa, Carneiro-Proietti Anna Bárbara de Freitas, Gonçalves Denise Ustch, Martins-Filho Olindo Assis

机构信息

Instituto René Rachou, FIOCRUZ-Minas, Belo Horizonte, Minas Gerais, Brazil; Hospital das Clínicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.

Hospital das Clínicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.

出版信息

Immunol Lett. 2021 Aug;236:68-77. doi: 10.1016/j.imlet.2021.05.009. Epub 2021 Jun 2.

DOI:10.1016/j.imlet.2021.05.009

PMID:34087263

Abstract

The impact of HIV co-infection on the plasma immunological biomarker profile of HTLV-1 infected patients was evaluated. The plasma levels of leukotrienes and chemokines/cytokines were quantified by ELISA and Cytometric Bead Array. A total of 138 volunteers were enrolled and divided into two subgroups ("HTLV-1(+)HIV(-)" and "HTLV-1(+)(HIV(+)"), which were categorized according to the HTLV-1-associated neurological disease (AS, pHAM and HAM). Reference controls were BD and HIV mono-infected patients. HAM(+) exhibited higher CD4 T-cell counts as compared to HIV+ mono-infected patients and lower HTLV-1 proviral load as compared to mono-infected HAM(-) patients. AS(+) exhibited higher levels of CysLT, CXCL8/IL-8 and lower levels of CCL5/RANTES as compared to AS(-). Increased levels of IL-6 and TNF with reduced levels of CXCL10/IP10 and CCL5/RANTES were observed in co-infected pHAM(+) as compared to mono-infected pHAM(-). HAM(+) patients revealed an increase in CXCL8/IL-8, CCL2/MCP-1, CXCL-10/IP-10, TNF and a decrease in IL-2 as compared to HAM(-) subgroup.

摘要

评估了HIV合并感染对HTLV-1感染患者血浆免疫生物标志物谱的影响。通过酶联免疫吸附测定（ELISA）和细胞计数微球阵列对血浆中白三烯和趋化因子/细胞因子水平进行定量。共招募了138名志愿者，并将其分为两个亚组（“HTLV-1(+)HIV(-)”和“HTLV-1(+)(HIV(+)”），根据HTLV-1相关神经系统疾病（AS、pHAM和HAM）进行分类。参考对照组为BD和HIV单感染患者。与HIV单感染患者相比，HAM(+)患者的CD4 T细胞计数更高；与HAM(-)单感染患者相比，其HTLV-1前病毒载量更低。与AS(-)相比，AS(+)患者的半胱氨酰白三烯（CysLT）、CXCL8/IL-8水平更高，CCL5/RANTES水平更低。与pHAM(-)单感染患者相比，合并感染的pHAM(+)患者中IL-6和TNF水平升高，CXCL10/IP10和CCL5/RANTES水平降低。与HAM(-)亚组相比，HAM(+)患者的CXCL8/IL-8、CCL2/MCP-1、CXCL-10/IP-10、TNF升高，IL-2降低。