School of Civil Engineering, Architecture and Urbanism - FEC, at UNICAMP (State University of Campinas), Avenida Albert Einstein, 951, Cidade Universitária "Zeferino Vaz", P.O. Box 6021, 13083-852 Campinas, SP, Brazil.
Laboratory of Environmental Microbiology, Department of Biology, Federal University of São Carlos, Rodovia João Leme dos Santos Km 110, Sorocaba, SP 18052-780, Brazil.
Bioresour Technol. 2021 Sep;336:125334. doi: 10.1016/j.biortech.2021.125334. Epub 2021 May 28.
The potential of three different kind of reactors and active biomass to be used as inoculum for nitrogen removal was verified. Sludge samples were collected from a Membrane Bioreactor (MBR), a previous tank of a Moving Bed Biofilm Reactor (MBBR) and a Trickling Filter (TF). Samples were compared according to bacterial activity in batch tests and their microbiology (16 s rRNA sequences). The microorganisms examined were: AOB, NOB, anammox bacteria and OHO. Results showed that the richest sample was from MBR (Chao value equals 581). However, the bacterial activity was greater in MBBR sample (q equals 0.002 mgN·mgVSS·h; q equals 0.001 mgN·mgVSS·h and q equals 10.0 mgN·mgVSS·h). Therefore, MBBR WWTP was shown to have the best inoculum and operating conditions for nitrogen conversion and removal. Besides, if aeration is provided as low as necessary for AOB to start the activity in denitrification tank, simultaneous partial nitrification, and denitrification (SPND) can occur.
验证了三种不同类型的反应堆和活性生物量作为脱氮接种物的潜力。从膜生物反应器 (MBR)、移动床生物膜反应器 (MBBR) 的前一个水箱和滴滤器 (TF) 中收集了污泥样本。根据批量测试中的细菌活性和微生物学(16s rRNA 序列)对样本进行了比较。检查的微生物有:AOB、NOB、厌氧氨氧化菌和 OHO。结果表明,MBR 的样本最丰富(Chao 值等于 581)。然而,MBBR 样本的细菌活性更大(q 等于 0.002mgN·mgVSS·h;q 等于 0.001mgN·mgVSS·h 和 q 等于 10.0mgN·mgVSS·h)。因此,MBBR WWTP 被证明具有最佳的接种物和氮转化和去除的操作条件。此外,如果曝气提供的氧气足以使 AOB 开始反硝化罐中的脱氮活性,就可以同时发生部分硝化和反硝化(SPND)。