Analytical Chemistry Department, Faculty of Pharmacy, Minia University, Minia, Egypt.
Analytical Chemistry Department, Faculty of Pharmacy, Minia University, Minia, Egypt; Department of Pharmacognosy and Pharmaceutical Chemistry, College of Pharmacy, Taibah University, Medinah, Saudi Arabia.
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Nov 15;261:119984. doi: 10.1016/j.saa.2021.119984. Epub 2021 May 21.
An innovative and sensitive spectrofluorimetric method has been developed for determination of 6-aminocaproic acid (ACA) in its pure form and its laboratory prepared tablets. The aim of this method is the reaction of ethyl acetoacetate and formaldehyde with the primary amino group presented in ACA as aimed in the Hantzsch reaction, this reaction resulted in formation of a yellow fluorescent dihydropyridine derivative that can be easily detected spectrofluorimetrically at 438 nm (excitation at 358 nm). At the optimum conditions of the reaction, the linear range was found to be (0.7-3.5 µg\mL) with limit of detection is 0.231 µg\mL and limit of quantitation is 0.700 µg\mL. The proposed method used for detection of ACA laboratory prepared tablets with average percentage 100.721 ± 0.701% without any interference from any excipients. This method used for in vitro determination of ACA in spiked human plasma with a percent mean recovery 99.874 ± 1.416%. In addition, the developed method used for determination of ACA in spiked human urine with percent mean recovery 100.314 ± 1.793%.
一种创新且灵敏的荧光分光光度法已被开发用于测定 6-氨基己酸（ACA）的纯品及其实验室制备的片剂。该方法的目的是使乙酰乙酸乙酯和甲醛与 ACA 中存在的伯氨基反应，如 Hantzsch 反应中所设想的那样，该反应生成一种黄色荧光二氢吡啶衍生物，可通过荧光分光光度法在 438nm（激发波长 358nm）处轻松检测到。在反应的最佳条件下，发现线性范围为（0.7-3.5μg/mL），检测限为 0.231μg/mL，定量限为 0.700μg/mL。该方法用于检测实验室制备的 ACA 片剂，平均百分含量为 100.721±0.701%，无任何赋形剂干扰。该方法用于人血浆中加标 ACA 的体外测定，平均回收率为 99.874±1.416%。此外，该方法还用于测定人尿中加标 ACA 的含量，平均回收率为 100.314±1.793%。
