草药提取物对大鼠坐骨神经显微手术后外周神经再生的影响。
Effect of herbal extracts on peripheral nerve regeneration after microsurgery of the sciatic nerve in rats.
机构信息
Department of Orthopedic Surgery, Kyung Hee University Hospital at Gangdong, School of Medicine, Kyung Hee University, 892 Dongnam-ro, Gangdong-gu, Seoul, Republic of Korea.
Department of Cardiology and Neurology, Kyung Hee University Hospital at Gangdong, College of Korean Medicine, Kyung Hee University, 892 Dongnam-ro, Gangdong-gu, Seoul, Republic of Korea.
出版信息
BMC Complement Med Ther. 2021 Jun 4;21(1):162. doi: 10.1186/s12906-021-03335-w.
BACKGROUND
Recent experimental studies using herbal extracts have shown the possibility of peripheral nerve regeneration. This study aimed to investigate the effects of herbal extracts on peripheral nerve regeneration in a rat sciatic nerve injury model.
METHODS
A total of 53 rats were randomly assigned to a control group or one of four experimental groups. In all rats, the sciatic nerve was completely severed and microscopic epineural end-to-end neurorrhaphy was performed. Normal saline (2 mL) was topically applied to the site of nerve repair in the control group, whereas four different herbal extracts - 2 mL each of Astragalus mongholicus Bunge, Coptis japonica (Thunb.) Makino, Aconitum carmichaelii Debeaux, or Paeonia lactiflora Pall. - were topically applied to the site of nerve repair in each experimental group. Nerve conduction studies were performed at an average of 11.9 weeks after the operation, and conduction velocity and proximal and distal amplitudes were measured. Biopsies were performed at an average of 13.2 weeks after the initial neurorrhaphy. The quality of nerve anastomosis and perineural adhesion to the surrounding soft tissues was macroscopically evaluated. The neuroma size at the site of the neurorrhaphy was microscopically measured, whereas the size of the scar tissue was evaluated relative to the diameter of the repaired nerve.
RESULTS
The nerve conduction study results showed the highest nerve conduction velocity in the experimental group that used the Coptis japonica (Thunb.) Makino extract and the highest proximal and distal amplitudes in the experimental group that used the Aconitum carmichaelii Debeaux extract. Macroscopic evaluations after the second operation showed that grade 2 perineural adhesion was found in 70.8% of rats. The mean neuroma size in the Coptis japonica (Thunb.) Makino, Aconitum carmichaelii Debeaux, and Paeonia lactiflora Pall. groups showed statistically significant decreases relative to the control group. The mean scar tissue formation index in the Paeonia lactiflora Pall. group showed a statistically significant decrease relative to the control group.
CONCLUSIONS
The peripheral nerve regeneration effect of the herbal extracts was confirmed through decreased neuroma and scar tissue formation.
背景
最近的实验研究表明，草药提取物具有促进周围神经再生的可能性。本研究旨在探讨草药提取物对大鼠坐骨神经损伤模型中周围神经再生的影响。
方法
将 53 只大鼠随机分为对照组和 4 个实验组。所有大鼠的坐骨神经均完全切断，并进行显微镜下的外膜端端神经吻合术。对照组在神经修复部位局部应用生理盐水（2 mL），而每个实验组则在神经修复部位局部应用 2 mL 的黄芪、黄连、乌头或白芍提取物。术后平均 11.9 周进行神经传导研究，测量传导速度和近端及远端幅度。初次神经吻合术后平均 13.2 周进行活检。宏观评估神经吻合部位的质量和周围软组织的神经粘连情况。显微镜下测量吻合部位神经瘤的大小，同时评估瘢痕组织相对于修复神经直径的大小。
结果
神经传导研究结果显示，使用黄连提取物的实验组神经传导速度最高，使用乌头提取物的实验组近端和远端幅度最高。第二次手术后的宏观评估显示，70.8%的大鼠出现 2 级神经粘连。黄连、乌头和白芍组的神经瘤平均大小与对照组相比均有显著减小。白芍组的瘢痕组织形成指数与对照组相比显著减小。
结论
通过减少神经瘤和瘢痕组织的形成，证实了草药提取物对周围神经再生的作用。
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