Hunan University of Chinese Medicine, Changsha, China.
Department of Proctology, The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, China.
J Clin Lab Anal. 2021 Jul;35(7):e23807. doi: 10.1002/jcla.23807. Epub 2021 Jun 5.
Circular RNA (circRNA) has been found to be involved in regulating tumor development. However, the roles and underlying mechanisms of circRNA in colorectal cancer (CRC) development remain unclear. In this study, we investigated the effects of hsa_circ_0031787 on CRC.a METHODS: Aberrant circRNA expression was explored by the Gene Expression Omnibus (GEO) database, and hsa_circ_0031787 was selected for further study. Hsa_circ_0031787 expression was determined in CRC tissues and cell lines by qRT-PCR. Cell proliferation was measured by Edu and colony formation assays. Cell invasion was tested by Transwell assays.
Hsa_circ_0031787 expression levels in CRC were significantly increased and correlated with advanced TNM stage and lymph node metastasis in CRC patients. Functional assays showed that hsa_circ_0031787 suppression reduced CRC cell proliferation and invasion in vitro and reduced tumor growth in vivo. Furthermore, hsa_circ_0031787 suppression reduced activation of the Wnt/β-catenin axis in CRC.
Our results showed that hsa_circ_0031787 may function as an oncogenic circRNA in CRC progression, thus providing a new potential therapeutic target.
环状 RNA（circRNA）已被发现参与调节肿瘤的发生。然而，circRNA 在结直肠癌（CRC）发展中的作用和潜在机制尚不清楚。在本研究中，我们研究了 hsa_circ_0031787 对 CRC 的影响。
通过基因表达综合数据库（GEO）数据库探讨异常 circRNA 的表达，并选择 hsa_circ_0031787 进行进一步研究。通过 qRT-PCR 检测 CRC 组织和细胞系中 hsa_circ_0031787 的表达。通过 Edu 和集落形成实验测定细胞增殖。通过 Transwell 测定法检测细胞侵袭。
CRC 中 hsa_circ_0031787 的表达水平显著升高，并与 CRC 患者的晚期 TNM 分期和淋巴结转移相关。功能测定表明，hsa_circ_0031787 的抑制减少了 CRC 细胞在体外的增殖和侵袭，并减少了体内肿瘤的生长。此外，hsa_circ_0031787 的抑制减少了 CRC 中 Wnt/β-catenin 轴的激活。
我们的研究结果表明，hsa_circ_0031787 可能在 CRC 进展中作为一种致癌性 circRNA 发挥作用，从而为新的潜在治疗靶点提供了依据。
