Daxing Research Institute, University of Science and Technology Beijing, Beijing 100083, China; 112 Lab, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China.
112 Lab, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China.
J Proteomics. 2021 Aug 15;245:104280. doi: 10.1016/j.jprot.2021.104280. Epub 2021 Jun 4.
Background The study of molecular profiling of dental pulp stem cells (DPSCs) and periodontal ligament stem cells (PDLSCs) contributes to understanding the high proliferation ability and multi-lineage differentiation potential. Objectives The aim of the study was to compare the protein abundance and specific markers of DPSCs and PDLSCs by protein profiles. Material and methods The DPSCs and PDLSCs extracted from the same tooth were lysed with 3 biological replicates and the protein was collected. Two-dimensional electrophoresis technology and TMT proteomics were used to separate and identify proteins. The data are available via ProteomeXchange with identifier PXD021997. The RT-qPCR detection of mRNA expression revealed a special marker for distinguishing two kinds of dental stem cells. Results Compared with PDLSCs, 962 differential proteins (DAPs) were up-regulated, and 127 were down-regulated in DPSCs. In the up-regulated DAPs, two high-scoring sub-networks were detected for neural-related molecules, which encode cell vesicle transport and mitochondrial energy transfer to regulate cell proliferation and secretion factors. A large number of cell adhesion molecules were distinguished among the highly expressed molecules of PDLSCs, supporting that stem cells provide cell attachment functions. It was interpreted ENPL, HS90A and HS90B were highly expressed in DPSCs, while CKB was highly abundant in PDLSCs. Another cell group confirmed that these molecules can be used as special biomarkers to identify and distinguish between DPSCs and PDLSCs. Conclusions This study can promote the basic research and clinical application of dental stem cells. Significance The high-throughput protein profiles were tested by combining two-dimensional gel proteomics and TMT-based proteomics. The proteomics of DPSCs and PDLSCs without individual difference demonstrated an accurate and comprehensive molecular expression profiles and interpretation of neural application potential, this study promotes the basic research of dental stem cells and clinical application.
背景 牙髓干细胞（DPSCs）和牙周膜干细胞（PDLSCs）的分子谱研究有助于了解其高增殖能力和多向分化潜能。目的 本研究旨在通过蛋白质谱比较 DPSCs 和 PDLSCs 的蛋白质丰度和特异性标志物。材料与方法 从同一颗牙齿中提取 DPSCs 和 PDLSCs，用 3 个生物学重复裂解细胞并收集蛋白质。采用二维电泳技术和 TMT 蛋白质组学分离和鉴定蛋白质。该数据可通过 ProteomeXchange 以标识符 PXD021997 获得。通过 RT-qPCR 检测 mRNA 表达揭示了区分两种牙源性干细胞的特殊标志物。结果 与 PDLSCs 相比，DPSCs 中有 962 个差异表达蛋白（DAPs）上调，127 个下调。在上调的 DAPs 中，检测到两个与神经相关分子的高分子网，这些分子编码细胞囊泡运输和线粒体能量传递，以调节细胞增殖和分泌因子。在 PDLSCs 高表达分子中区分出大量的细胞黏附分子，支持干细胞提供细胞附着功能。解释为 DPSCs 中高度表达的 ENPL、HS90A 和 HS90B，而 CKB 在 PDLSCs 中丰度较高。另一个细胞群证实这些分子可作为特殊生物标志物，用于鉴定和区分 DPSCs 和 PDLSCs。结论 本研究可促进牙源性干细胞的基础研究和临床应用。意义 本研究采用二维凝胶蛋白质组学和基于 TMT 的蛋白质组学相结合，对 DPSCs 和 PDLSCs 的高通量蛋白质谱进行了测试。无个体差异的 DPSCs 和 PDLSCs 的蛋白质组学展示了准确而全面的分子表达谱，并对神经应用潜力进行了阐释，本研究促进了牙源性干细胞的基础研究和临床应用。
