Department of Population Health and Pathobiology, College of Veterinary Medicine, Raleigh, NC 27607, USA.
Department of Population Health and Pathobiology, College of Veterinary Medicine, Raleigh, NC 27607, USA.
Vet Immunol Immunopathol. 2021 Jul;237:110277. doi: 10.1016/j.vetimm.2021.110277. Epub 2021 Jun 1.
Cytokine transcripts were evaluated chronologically in the brain and in the eye of chickens infected with the very virulent plus Marek's disease virus (vv + MDV) strain 648A. Brain and eye samples were collected from chickens that were either suffering from transient paralysis (TP) (11 days post inoculation, dpi) or had completely recovered from TP but started developing clinical signs of persistent neurological disease (PND) (18-31 dpi). Results obtained from samples collected at 11 dpi are referred as EL (early lesions) and results obtained from samples collected at later times (18-31 dpi) are referred as LL (late lesions). Marked differences were found in the cytokine transcripts in brain and eye. While proinflammatory cytokines (IL-1β, IL-8, IL-18), iNOS, IFN-α, IFN-γ, and IL-15 were upregulated in the brain during EL and LL, only IL-8 and IFN-γ were upregulated in the eye at both times (EL and LL). The two evaluated viral transcripts (gB and meq) were found in both eye and brain during EL and LL. Levels of the two viral transcripts evaluated were higher at LL than at EL in both brain and eye. No differences were found in any of the viral transcripts between eye and brain during EL. However, during the LL, the levels of meq transcripts were higher in the eye than in the brain. Our results suggest that MDV elicits different immune responses in the brain and in the eye of infected chickens. Because immune responses in the eye of chickens have been poorly studied, further studies on the pathogenesis of MDV in the eye could greatly contribute to our knowledge on the chicken eye immunity.
在感染非常强毒力+马立克氏病病毒（vv + MDV）株 648A 的鸡的大脑和眼睛中，按时间顺序评估细胞因子转录物。从患有短暂麻痹（TP）的鸡（接种后 11 天，dpi）或已经从 TP 完全康复但开始出现持续性神经疾病（PND）临床症状的鸡中收集大脑和眼睛样本（18-31 dpi）。在 11 dpi 收集的样本中获得的结果称为 EL（早期病变），在稍后时间（18-31 dpi）收集的样本中获得的结果称为 LL（晚期病变）。在大脑和眼睛中发现细胞因子转录物存在明显差异。虽然促炎细胞因子（IL-1β、IL-8、IL-18）、iNOS、IFN-α、IFN-γ和 IL-15 在 EL 和 LL 期间在大脑中上调，但在眼睛中仅在两个时间点（EL 和 LL）上调了 IL-8 和 IFN-γ。在 EL 和 LL 期间，在眼睛和大脑中均发现了两种评估的病毒转录物（gB 和 meq）。在大脑和眼睛中，两种病毒转录物的评估水平在 LL 时均高于 EL。在 EL 期间，在眼睛和大脑之间未发现任何病毒转录物存在差异。然而，在 LL 期间，meq 转录物的水平在眼睛中高于大脑。我们的研究结果表明，MDV 在感染鸡的大脑和眼睛中引发不同的免疫反应。由于鸡眼睛中的免疫反应研究甚少，因此进一步研究 MDV 在眼睛中的发病机制可能会极大地促进我们对鸡眼睛免疫的认识。
