SUMY STATE UNIVERSITY, SUMY, UKRAINE.
Wiad Lek. 2021;74(5):1200-1203.
The aim: The objective of the study was to analyze the frequency of Arg16Gly polymorphism in the β2 -adrenoceptor (β2 -АR) gene in patients with bronchial asthma (BA) and to assess the association of the polymorphism with BA risk.
Materials and methods: We examined 553 BA patients and 95 apparently healthy individuals. Arg16Gly polymorphism in the β2 -АR gene (rs1042713) was determined using polymerase chain reaction-restriction fragment length polymorphism analysis. Statistical analysis of obtained results was performed using SPSS-17 program.
Results: It was established that distribution of Arg/Arg, Arg/Gly, and Gly/Gly genotypes for Arg16Gly polymorphism in the β2 -АR gene was 44.2%, 40.0%, 15.8% in the control group vs. 31.3%; 45.7% and 23.0 among BA patients, respectively (χ2 = 6.59; р = 0.037). No significant difference was observed with regards to the distribution of genotypes for Arg16Gly polymorphism in the β2 -АR gene in men and women controls (χ2 = 4.05; р = 0.13) and BA patients (χ2 = 4.34; р = 0.11). BA risk was 1.74 times higher in the minor allele carriers (Arg/Gly + Gly/Gly genotypes) for Arg16Gly polymorphism in the β2 -АR gene.
Conclusions: Analysis of Arg16Gly polymorphic variants in the β2-AR gene showed a statistically significant difference in the distribution of Arg/Arg, Arg/Gly, and Gly/Gly genotypes in patients with BA and apparently healthy individuals due to the higher frequency of Arg/Arg genotype in controls and higher frequency of Gly/Gly genotype in patients with asthma. No difference with regard to gender was found in the distribution of genotypes.
分析β2-肾上腺素能受体（β2-AR）基因 Arg16Gly 多态性在支气管哮喘（BA）患者中的频率，并评估该多态性与 BA 风险的相关性。
我们检查了 553 例 BA 患者和 95 名健康个体。使用聚合酶链反应-限制性片段长度多态性分析（PCR-RFLP）确定β2-AR 基因（rs1042713）的 Arg16Gly 多态性。使用 SPSS-17 程序对获得的结果进行统计学分析。
研究发现，β2-AR 基因 Arg16Gly 多态性的 Arg/Arg、Arg/Gly 和 Gly/Gly 基因型在对照组中的分布分别为 44.2%、40.0%和 15.8%，而在 BA 患者中分别为 31.3%、45.7%和 23.0%（χ2 = 6.59；р = 0.037）。在男性和女性对照组（χ2 = 4.05；р = 0.13）和 BA 患者（χ2 = 4.34；р = 0.11）中，β2-AR 基因 Arg16Gly 多态性的基因型分布无显著差异。携带 Arg16Gly 多态性的β2-AR 基因的次要等位基因（Arg/Gly+Gly/Gly 基因型）的 BA 风险增加 1.74 倍。
β2-AR 基因 Arg16Gly 多态性分析显示，由于对照组 Arg/Arg 基因型频率较高，而哮喘患者 Gly/Gly 基因型频率较高，因此 BA 患者和健康个体的 Arg/Arg、Arg/Gly 和 Gly/Gly 基因型分布存在统计学显著差异。基因型分布在性别方面无差异。
