Elder G A, Major E O
Infectious Diseases Branch, DIR, National Institute of Neurological and Communicative Disorders and Stroke, Bethesda, MD 20892.
Brain Res. 1988 Jul 1;470(1):146-50. doi: 10.1016/0165-3806(88)90211-8.
To compare rat and human glial development, we examined the cellular composition of human fetal brain in short-term cultures and fresh cell suspensions from fetal ages ranging from 7 to 16 weeks, utilizing the cell type-specific markers which define glial subsets in rats. Here we report that unlike the early rat central nervous system (CNS), 7-10 week human fetal brain contains mostly astrocytes that can be characterized as type II rather than type I. Type I astrocytes become more prevalent in 16-week gestational age human brain. Although cells morphologically and immunocytochemically similar to the rat 02-A cell are found in human fetal brain and spinal cord, these cells were not induced to express galactocerebroside in serum-free media and did not have vimentin-containing intermediate filaments as do rat 02-A cells. These results suggest that functional differences may exist between rat type I and type II astrocytes and phenotypically similar cells found in humans.
为了比较大鼠和人类神经胶质细胞的发育情况，我们利用定义大鼠神经胶质细胞亚群的细胞类型特异性标志物，对来自7至16周胎儿的短期培养物和新鲜细胞悬液中的人类胎儿大脑细胞组成进行了检测。在此我们报告，与早期大鼠中枢神经系统（CNS）不同，7至10周的人类胎儿大脑主要包含可被归类为II型而非I型的星形胶质细胞。I型星形胶质细胞在孕16周的人类大脑中更为普遍。尽管在人类胎儿大脑和脊髓中发现了形态和免疫细胞化学上与大鼠02 - A细胞相似的细胞，但这些细胞在无血清培养基中未被诱导表达半乳糖脑苷脂，也没有像大鼠02 - A细胞那样含有波形蛋白的中间丝。这些结果表明，大鼠I型和II型星形胶质细胞与人类中表型相似的细胞之间可能存在功能差异。
