Green Separation Engineering Laboratory, SASTRA Deemed to be University, Thanjavur, Tamil Nadu 613401, India.
Green Separation Engineering Laboratory, SASTRA Deemed to be University, Thanjavur, Tamil Nadu 613401, India.
Bioresour Technol. 2021 Sep;336:125357. doi: 10.1016/j.biortech.2021.125357. Epub 2021 Jun 2.
Wastewater effluent from meat processing industries are enriched with nutrients but remain underutilized. Therefore, an efficient method was administered by employing this resource for the cultivation of Porphyreum cruentium. The cultured biomass was exposed to a one-step disruption and selective purification method using a protic deep eutectic solvent to obtain a commercially significant pigment, R-Phycoerythrin (R-PE). Six protic deep eutectic solvents (pDES) were synthesized and their thermophysical activity determined. A synergy between microwave and cavitation (MACE-DLPME) has been achieved for the effective recovery of these membrane proteins. The addition of pDES provides the selective medium for the concentration of R-PE. Optimization of the MACE-DLPME method yields 95.9% (w/w) of R-PE. Preparative size exclusion chromatography resulted in (28 µg/g) of R-PE. Further ultra-purification by anion exchange chromatography enhances the purity fold of R-PE to 125. The resulting ultrapure fraction exhibits enhanced anti-platelet activity (1.56 mg/g ascorbic acid equivalent).
肉类加工行业的废水富含营养物质，但尚未得到充分利用。因此，采用了一种有效的方法，利用这一资源来培养血褐藻。培养的生物质经过一步破碎和选择性纯化方法，使用质子亲液共晶溶剂来获得具有商业意义的色素，藻红蛋白（R-PE）。合成了六种质子亲液共晶溶剂（pDES），并测定了它们的热物理性质。微波和空化（MACE-DLPME）的协同作用实现了这些膜蛋白的有效回收。pDES 的添加为 R-PE 的浓缩提供了选择性介质。MACE-DLPME 方法的优化得到了 95.9%（w/w）的 R-PE。制备尺寸排阻色谱得到了 28μg/g 的 R-PE。进一步通过阴离子交换色谱进行超纯纯化，将 R-PE 的纯度提高到 125 倍。得到的超纯级分表现出增强的抗血小板活性（相当于 1.56mg/g 抗坏血酸）。
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