Department of Pediatric Endocrinology, Izmir KatipÇelebi University, Faculty of Medicine, İzmir, Turkey.
Department of Pediatric Endocrinology, Dokuz Eylül University, Faculty of Medicine, İzmir, Turkey.
Int J Obes (Lond). 2021 Sep;45(9):2064-2073. doi: 10.1038/s41366-021-00876-5. Epub 2021 Jun 5.
BACKGROUND/OBJECTIVES: In recent years, oxytocin (OXT) and polymorphisms in the oxytocin receptor (OXTR) gene have been reported to play roles in obesity pathogenesis. However, there was no study evaluating OXTR gene variants in childhood obesity. The aim of the study was to investigate the relation of OXTR gene polymorphisms and serum OXT levels with metabolic and anthropometric parameters in obese and healthy adolescents.
SUBJECTS/METHODS: The study was a multi-centered case-control study, which was conducted on obese and healthy adolescents aged between 12 and 17 years. Serum OXT and leptin levels were measured, and OXTR gene variants were studied by qPCR (rs53576) and RFLP (rs2254298) methods.
A total of 250 obese and 250 healthy adolescents were included in this study. In the obese group, serum OXT level was lower and leptin level was higher than the control group. In the obese group, frequencies of homozygous mutant (G/G) and heterozygous (A/G) genotypes for rs53576 polymorphism were higher than the control group. Homozygous mutant(G/G) and heterozygous (A/G) genotypes for rs53576 polymorphism were found to increase the risk of obesity compared to the wild type (A/A) genotype [OR = 6.05 and OR = 3.06; p < 0.001, respectively]. In patients with homozygous mutant (G/G) and heterozygous (A/G) genotype for rs53576 polymorphism, serum OXT levels were lower than the wild type (A/A) genotype. In the obese group, hyperphagia score was higher than the control group and correlated negatively with serum OXT level.
This study revealed that low serum OXT level, which is associated with hyperphagia may be an underlying cause for obesity in adolescents. For rs53576 polymorphism of the OXTR gene, obesity risk is higher in patients with homozygous mutant(G/G) and heterozygous(A/G)genotypes.
背景/目的：近年来，催产素（OXT）和催产素受体（OXTR）基因的多态性被报道在肥胖发病机制中起作用。然而，目前还没有研究评估 OXTR 基因变异与儿童肥胖的关系。本研究旨在探讨 OXTR 基因多态性与血清 OXT 水平与肥胖和健康青少年代谢和人体测量参数的关系。
受试者/方法：该研究是一项多中心病例对照研究，纳入了 12 至 17 岁的肥胖和健康青少年。测量血清 OXT 和瘦素水平，并通过 qPCR（rs53576）和 RFLP（rs2254298）方法研究 OXTR 基因变异。
本研究共纳入 250 名肥胖和 250 名健康青少年。在肥胖组中，血清 OXT 水平低于对照组，瘦素水平高于对照组。在肥胖组中，rs53576 多态性的纯合突变（G/G）和杂合（A/G）基因型的频率高于对照组。与野生型（A/A）基因型相比，rs53576 多态性的纯合突变（G/G）和杂合（A/G）基因型增加了肥胖的风险[OR=6.05 和 OR=3.06；p<0.001]。在 rs53576 多态性纯合突变（G/G）和杂合（A/G）基因型的患者中，血清 OXT 水平低于野生型（A/A）基因型。在肥胖组中，摄食评分高于对照组，与血清 OXT 水平呈负相关。
本研究表明，与摄食过多相关的低血清 OXT 水平可能是青少年肥胖的一个潜在原因。对于 OXTR 基因的 rs53576 多态性，纯合突变（G/G）和杂合（A/G）基因型患者的肥胖风险更高。
