Kondratiev Y S, Brukhansky G V, Andreeva I V, Skavronskaya A G
Mol Gen Genet. 1977 Dec 30;158(2):211-4. doi: 10.1007/BF00268315.
The UV-sensitivity of wild type Salmonella strains has been compared to that of wild type E. cole and its UV-sensitive mutants. Many wild type Salmonella strains are 4-5 times more sensitive than wild type E. coli and their inactivation curves is similar to that for E. coli with a mutation in the polA gene. Alkaline sucrose gradient centrifugation has shown a deficiency of these strains in normal excision repair of UV-damaged DNA. This deficiency is not a Salmonella genus feature because one strain as resistant as wild type E. coli was found. This resistant strain showed normal excision repair in alkaline sucrose gradient centrifugation experiments. The possible influence of plasmids and mutations in repair genes on the ability of Salmonella to repair UV-damages DNA is discussed.
已将野生型沙门氏菌菌株的紫外线敏感性与野生型大肠杆菌及其紫外线敏感突变体的敏感性进行了比较。许多野生型沙门氏菌菌株的敏感性比野生型大肠杆菌高4至5倍，其失活曲线与具有polA基因突变的大肠杆菌相似。碱性蔗糖梯度离心表明，这些菌株在紫外线损伤DNA的正常切除修复方面存在缺陷。这种缺陷并非沙门氏菌属的特征，因为发现了一株与野生型大肠杆菌一样具有抗性的菌株。在碱性蔗糖梯度离心实验中，该抗性菌株显示出正常的切除修复。本文还讨论了质粒和修复基因突变对沙门氏菌修复紫外线损伤DNA能力的可能影响。
