State Key Laboratory of Veterinary Etiological Biology, Office International des Epizootie (OIE)/China National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
School of Animal Science, Yangtze University, Jingzhou, China.
Front Immunol. 2021 May 20;12:616402. doi: 10.3389/fimmu.2021.616402. eCollection 2021.
In addition to ribosomal protein synthesis and protein translation, ribosomal proteins also participate in tumorigenesis and tumor progression, immune responses, and viral replication. Here, we show that ribosomal protein L13 (RPL13) participates in the antiviral immune response induced by foot-and-mouth disease virus (FMDV), inhibiting FMDV replication. The overexpression of RPL13 promoted the induction and activation of the promoters of the nuclear factor-κB (NF-κB) and interferon-β (IFN-β) genes, and the expression and protein secretion of the antiviral factor IFN-β and proinflammatory cytokine interleukin-6 (IL-6). The knockdown of RPL13 had the opposite effects. We also found that the FMDV 3C protease interacts with RPL13, and that its activity reduces the expression of RPL13, thus antagonizing the RPL13-mediated antiviral activity. This study extends our knowledge of the extraribosomal functions of ribosomal proteins and provides new scientific information on cellular antiviral defenses and virus-antagonizing mechanisms.
除了核糖体蛋白合成和蛋白质翻译，核糖体蛋白还参与肿瘤发生和肿瘤进展、免疫反应和病毒复制。在这里，我们表明核糖体蛋白 L13（RPL13）参与口蹄疫病毒（FMDV）诱导的抗病毒免疫反应，抑制 FMDV 复制。RPL13 的过表达促进了核因子-κB（NF-κB）和干扰素-β（IFN-β）基因启动子的诱导和激活，以及抗病毒因子 IFN-β和促炎细胞因子白细胞介素-6（IL-6）的表达和蛋白分泌。RPL13 的敲低则产生相反的效果。我们还发现，口蹄疫病毒 3C 蛋白酶与 RPL13 相互作用，其活性降低了 RPL13 的表达，从而拮抗了 RPL13 介导的抗病毒活性。本研究扩展了我们对手核糖体蛋白的核糖体蛋白的非核糖体功能的认识，并为细胞抗病毒防御和病毒拮抗机制提供了新的科学信息。
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