Sen A, Yang P W, Mantsch H H, Hui S W
Department of Biophysics, Roswell Park Memorial Institute, Buffalo, NY 14263.
Chem Phys Lipids. 1988 Jun;47(2):109-16. doi: 10.1016/0009-3084(88)90079-5.
The structure of phosphatidylethanolamine in pure dry hexane was studied. Viscosity measurements show that the hexane solution of PE has a very high viscosity, while freeze fracture electron microscopy revealed extensive fibre-like structures. These extended structures are disrupted by the addition of small amounts of water or organic solvents which are capable of hydrogen-bonding. The Fourier transform infrared spectra of the lipid solutions in dry and hydrated hexane show considerable differences in the phosphate and ethanolamine absorption bands, and demonstrate that the viscous fibre-like structures formed by phosphatidylethanolamine in dry hexane consist of extended intermolecular hydrogen-bonds, similar to those found in the solid lipid, with the ammonium group as the hydrogen-donor and the phosphate group as the hydrogen-acceptor. The high viscosity is not observed in hexane solution of phosphatidylcholine.
对纯干己烷中磷脂酰乙醇胺的结构进行了研究。粘度测量表明，PE的己烷溶液具有非常高的粘度，而冷冻断裂电子显微镜显示出广泛的纤维状结构。添加少量能够形成氢键的水或有机溶剂会破坏这些伸展结构。干燥和水合己烷中脂质溶液的傅里叶变换红外光谱显示，磷酸盐和乙醇胺吸收带存在显著差异，并表明磷脂酰乙醇胺在干己烷中形成的粘性纤维状结构由伸展的分子间氢键组成，类似于在固体脂质中发现的氢键，其中铵基团作为氢供体，磷酸基团作为氢受体。在磷脂酰胆碱的己烷溶液中未观察到高粘度。
