Scott D M, Kumar S, Barnes M J
Strangeways Research Laboratory, Worts Causeway, Cambridge, U.K.
Cell Biochem Funct. 1988 Jul;6(3):209-15. doi: 10.1002/cbf.290060310.
Cultured capillary endothelial cells, derived from bovine brain, and maintained on a plastic substratum synthesized predominantly interstitial collagens of which approximately 75 per cent were secreted into the medium. When grown on a native hydrated collagen type I gel, although no marked alteration in the 'collagen synthetic pattern' was observed, the overall level of collagen synthesis was increased by approximately 100 per cent. More dramatic, however, was the alteration in the distribution of these molecules between medium and cell layer. Interstitial collagens produced by cells grown on collagen gels were almost exclusively associated with the cell layer or collagenous gel. These studies, thus, demonstrate that an extracellular matrix may exert a considerable influence on the cellular synthetic activities and possibly cellular polarity of capillary endothelial cells.
从牛脑分离得到的培养毛细血管内皮细胞，在塑料基质上生长时，主要合成间质胶原，其中约75%分泌到培养基中。当在天然水合I型胶原凝胶上生长时，尽管未观察到“胶原合成模式”有明显改变，但胶原合成的总体水平增加了约100%。然而，更显著的是这些分子在培养基和细胞层之间分布的改变。在胶原凝胶上生长的细胞产生的间质胶原几乎完全与细胞层或胶原凝胶相关。因此，这些研究表明，细胞外基质可能对毛细血管内皮细胞的细胞合成活性以及可能的细胞极性产生相当大的影响。
