Stein C, Classen H G, Schwedt G
Department of Pharmacology and Toxicology of Nutrition, University of Hohenheim, FRG.
Clin Chim Acta. 1988 Jul 15;175(2):167-73. doi: 10.1016/0009-8981(88)90006-x.
A highly sensitive and specific ion-pair chromatographic method is presented for the simultaneous determination of nitrite (NO2-) and nitrate (NO3-) in plasma. The detection limits are 2 mumol NO2-/l and 8 mumol NO3-/l with recovery rates of 98-99%. The applicability of this method to clinical medicine is demonstrated using NO2- -loaded rats as a biological model: following intravenous infusion or intragastric application of NO2-, blood levels of NO2-, NO3- and methemoglobin are measured during 2-64 min. The kinetic data obtained reflect the complex metabolism of NO2- and NO3- in living organisms.
本文介绍了一种高灵敏度和特异性的离子对色谱法，用于同时测定血浆中的亚硝酸盐（NO2-）和硝酸盐（NO3-）。检测限分别为2 μmol NO2-/l和8 μmol NO3-/l，回收率为98-99%。以亚硝酸钠负荷大鼠作为生物学模型，证明了该方法在临床医学中的适用性：静脉输注或胃内给予亚硝酸钠后，在2-64分钟内测定血液中亚硝酸根、硝酸根和高铁血红蛋白的水平。获得的动力学数据反映了生物体中亚硝酸根和硝酸根的复杂代谢情况。
