Rajagopal Deepa, Tian Linhua, Xiong Shiqiu, Wang Lili, Campbell Jonathan, Saraiva Luisa, Vessillier Sandrine
Biotherapeutics Division, National Institute for Biological Standards and Control (NIBSC), Blanche Lane, South Mimms, Potters Bar, Hertfordshire, EN6 3QG, UK.
Biosystems and Biomaterials Division, National Institute of Standards and Technology (NIST), Gaithersburg, MD, 20899, USA.
J Immunol Regen Med. 2020 Sep;9. doi: 10.1016/j.regen.2020.100032.
Accurate determination of cellular subsets that secrete particular cytokine(s) is a significant parameter for functional characterization of an immunological response. The present study was conducted to develop a method for simultaneous measurement of intracellular cytokine positive CD4 and CD8 positive T lymphocytes in a single tube, with a no-wash protocol. We report here the development of a simplified, rapid procedure for precise enumeration of cytokine positive T lymphocytes using BD Trucount tubes. This single step protocol for accurate enumeration of cytokine positive T lymphocytes, will allow for better characterization of immune cell phenotype and function.
准确测定分泌特定细胞因子的细胞亚群是免疫反应功能特征化的一个重要参数。本研究旨在开发一种在单管中同时测量细胞内细胞因子阳性CD4和CD8阳性T淋巴细胞的方法，且无需洗涤步骤。我们在此报告了一种使用BD Trucount管精确计数细胞因子阳性T淋巴细胞的简化、快速程序。这种用于精确计数细胞因子阳性T淋巴细胞的单步方案，将有助于更好地表征免疫细胞表型和功能。
