Institute for Molecules and Materials, Radboud University, Nijmegen, The Netherlands.
Magn Reson Chem. 2021 Dec;59(12):1236-1243. doi: 10.1002/mrc.5182. Epub 2021 Jun 6.
Recent developments in para-hydrogen-induced polarization (PHIP) methods allow the nuclear magnetic resonance (NMR) detection of specific classes of compounds, down to sub-micromolar concentration in solution. However, when dealing with complex mixtures, signal resolution requires the acquisition of 2D PHIP-NMR spectra, which often results in long experimental times. This strongly limits the applicability of these 2D PHIP-NMR techniques in areas in which high-throughput analysis is required. Here, we present a combination of fast acquisition and nonuniform sampling that can afford a 10-fold reduction in measuring time without compromising the spectral quality. This approach was tested on a mixture of substrates at micromolar concentration, for which a resolved 2D PHIP spectrum was acquired in less than 3 min.
近年来，反氢诱导极化（PHIP）方法的发展使得特定类别的化合物可以通过核磁共振（NMR）检测到，其在溶液中的浓度低至亚微米级。然而，在处理复杂混合物时，信号分辨率需要采集二维 PHIP-NMR 谱，这通常会导致实验时间延长。这极大地限制了这些二维 PHIP-NMR 技术在需要高通量分析的领域中的应用。在这里，我们提出了一种快速采集和非均匀采样的组合方法，可以在不影响光谱质量的情况下将测量时间缩短 10 倍。该方法在微摩尔浓度的底物混合物上进行了测试，在不到 3 分钟的时间内获得了分辨率较高的二维 PHIP 谱。
