Biochemical Conversion Department, DBFZ Deutsches Biomasseforschungszentrum gemeinnützige GmbH (German Biomass Research Centre), Torgauer Straße 116, 04347 Leipzig, Germany.
Department of Environmental Microbiology, Helmholtz Centre for Environmental Research - UFZ, 04318 Leipzig, Germany.
Environ Sci Technol. 2021 Jun 15;55(12):8258-8266. doi: 10.1021/acs.est.0c07320. Epub 2021 Jun 7.
Anaerobic digestion (AD) and microbial electrochemical technologies (MET) can be combined in manifold ways. Recent studies show negative influences of AD effluents on the performance of pre-grown spp.-dominated biofilm anodes. In this study, it was investigated how such biofilm anodes are affected by AD effluents. Therefore, experiments using AD effluents in different concentrations (0-100%) in combination with biofilms of different ages were performed. Furthermore, the activity of methanogens was inhibited and minimized by application of 2-bromoethanesulfonate (2-BES) and microfiltration, respectively. Biofilms pre-grown for 5 weeks show higher resistance against AD effluents compared to biofilms pre-grown for only 3 weeks. Nevertheless, adaptation of biofilms to AD effluents was not successful. Biofilm activity in terms of coulombic efficiency and maximum current density () dropped by factor 32.2 ± 3.2 and 38.9 ± 8.4, respectively. The application of 2-BES and microfiltration had positive effects on the biofilm activity. The results support the assumption that methanogens or further compounds not studied here, for example, protozoans, which may have been inhibited or removed by 2-BES application or microfiltration, have an immediate influence on the stability of spp.-dominated biofilms and may limit their practical application in AD environments.
厌氧消化 (AD) 和微生物电化学技术 (MET) 可以以多种方式结合。最近的研究表明，AD 流出物对预先生长的 spp. 为主的生物膜阳极的性能有负面影响。在这项研究中，研究了 AD 流出物如何影响这种生物膜阳极。因此，使用不同浓度（0-100%）的 AD 流出物并结合不同年龄的生物膜进行了实验。此外，通过应用 2-溴乙磺酸盐（2-BES）和微滤分别抑制和最小化产甲烷菌的活性。与仅预生长 3 周的生物膜相比，预生长 5 周的生物膜对 AD 流出物具有更高的抵抗力。然而，生物膜对 AD 流出物的适应并不成功。生物膜的库仑效率和最大电流密度（）分别下降了 32.2±3.2 和 38.9±8.4。2-BES 和微滤的应用对生物膜活性有积极影响。结果支持这样的假设，即产甲烷菌或进一步未在此处研究的化合物，例如原生动物，可能已被 2-BES 应用或微滤抑制或去除，它们会立即影响 spp. 为主的生物膜的稳定性，并可能限制它们在 AD 环境中的实际应用。
