Wang L C, Belke D, Jourdan M L, Lee T F, Westly J, Nurnberger F
Zoology Department, University of Alberta, Edmonton, Canada.
Cryobiology. 1988 Aug;25(4):355-62. doi: 10.1016/0011-2240(88)90043-0.
Even though the existence of the blood-borne "hibernation induction trigger" has been reported in the 13-lined ground squirrel, transfusion of plasma from hibernating rodents with other hibernating species as the recipients failed to induce the occurrence of summer hibernation. In order to verify whether the response to the "trigger" substance is species specific, the present study was carried out to compare the effect of plasma from hibernating Richardson's ground squirrels on the incidence of summer hibernation in both juvenile Richardson's and adult 13-lined ground squirrels. In two series of experiments, 13-lined ground squirrels entered hibernation quite readily independent of the treatment. The rate of occurrence of hibernation ranged from 78% after sham injection to 86% after warm saline, fresh summer active plasma, and fresh hibernating plasma, respectively. There were no differences in the number of hibernation bouts and the number of days in hibernation after each treatment. In contrast, none of the juvenile Richardson's ground squirrels entered hibernation after any of the treatments up to the end of the 8-week observation period. These results not only argue against the existence of blood-borne "trigger" substance, at least in the Richardson's ground squirrel, but also caution against the use of the 13-lined ground squirrel as a standard test animal for the bioassay of the "trigger" substance.
尽管在三线地松鼠中已报道存在血源性“冬眠诱导触发因子”，但将冬眠啮齿动物的血浆输给其他冬眠物种作为受体时，未能诱导夏眠的发生。为了验证对“触发”物质的反应是否具有物种特异性，本研究进行了比较，以探究冬眠的里氏地松鼠的血浆对幼年里氏地松鼠和成年三线地松鼠夏眠发生率的影响。在两个系列的实验中，三线地松鼠很容易进入冬眠状态，与处理方式无关。冬眠发生率从假注射后的78%到温盐水、新鲜夏季活跃期血浆和新鲜冬眠期血浆处理后的86%不等。每种处理后，冬眠发作次数和冬眠天数均无差异。相比之下，在长达8周的观察期结束时，任何处理后的幼年里氏地松鼠均未进入冬眠状态。这些结果不仅反驳了血源性“触发”物质的存在，至少在里氏地松鼠中是这样，而且还警示不要将三线地松鼠用作“触发”物质生物测定的标准实验动物。
