Niederkorn J Y, Knisely T L
Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas 75235-9057.
Curr Eye Res. 1988 May;7(5):515-26. doi: 10.3109/02713688809031806.
Spontaneous rejection of syngeneic intraocular P91 mastocytoma occurred by a T cell-dependent immune process that produced extensive, irreparable damage to normal ocular tissue indicative of a delayed-type hypersensitivity (DTH) lesion. We investigated the contribution of other effector modalities in this tumor resolution. Antibody was not responsible for either tumor rejection of phthisis of the tumor-containing eye since passively transferred hyperimmune serum failed to produce either tumor rejection or the characteristic ocular lesions. Moreover, the destructive pattern of tumor rejection occurred in splenectomized hosts which had depressed anti-P91 serum antibody and in a congenic strain (B10.D2oSn) lacking the C5 complement component. The likelihood that tumor necrosis factor (TNF) was involved in this pathogenesis was also ruled out by the observation that P91 tumor cells were not susceptible to lysis by recombinant TNF. A different pattern of tumor resolution was observed when the opposite eye was challenged with tumor. In this case, the tumor was rejected without damaging any major ocular structures. Histological analysis demonstrated a mononuclear cellular infiltrate which was identified by immunohistology to be Thy 1+, Lyt 2+. Thus, although both forms of cell-mediated immunity are available to the host, only one predominated in each eye. These results suggest that unique immunological control mechanisms influence the outcome of tumor resolution in the eye.
同基因眼内P91肥大细胞瘤的自发排斥是由T细胞依赖性免疫过程介导的，该过程对正常眼组织造成了广泛的、不可修复的损伤，提示为迟发型超敏反应（DTH）病变。我们研究了其他效应方式在该肿瘤消退过程中的作用。抗体对含瘤眼的肿瘤排斥或眼球痨均无作用，因为被动转移的超免疫血清未能引起肿瘤排斥或特征性的眼部病变。此外，肿瘤排斥的破坏模式发生在脾切除的宿主中，这些宿主的抗P91血清抗体水平降低，且发生在缺乏C5补体成分的同基因品系（B10.D2oSn）中。P91肿瘤细胞对重组TNF不敏感这一观察结果也排除了肿瘤坏死因子（TNF）参与该发病机制的可能性。当对侧眼用肿瘤进行攻击时，观察到了不同的肿瘤消退模式。在这种情况下，肿瘤被排斥，而未损伤任何主要的眼部结构。组织学分析显示有单核细胞浸润，免疫组织化学鉴定为Thy 1+、Lyt 2+。因此，尽管宿主可利用两种形式的细胞介导免疫，但每只眼中只有一种占主导。这些结果表明，独特的免疫控制机制影响眼内肿瘤消退的结果。
