大麻的主要成分 Δ9-四氢大麻酚 (THC) 和大麻二酚 (CBD) 在 Sprague Dawley 大鼠跟腱手术修复模型中的生物力学效应：一项初步研究。

Biomechanical Effects of Δ9-Tetrahydrocannabinol (THC) and Cannabidiol (CBD), the Major Constituents of Cannabis, in a Sprague Dawley Rat Achilles Tendon Surgical Repair Model: A Pilot Study.

机构信息

Department of Orthopedics and Rehabilitation, Center for Orthopedic Research and Translational Sciences (CORTS), Penn State Hershey Medical Center, Hershey, Pennsylvania, USA.

Penn State Hershey Medical Center, Hershey, Pennsylvania, USA.

出版信息

Am J Sports Med. 2021 Jul;49(9):2522-2527. doi: 10.1177/03635465211016840. Epub 2021 Jun 7.

DOI:10.1177/03635465211016840

PMID:34097540

Abstract

BACKGROUND

The use of cannabis is common among athletes and the US population at large. Cannabinoids are currently being evaluated as alternatives to opioid medications for chronic pain management. However, the effects of recreational and/or medical use of delta-9-tetrahydrocannabinol (THC) and cannabidiol (CBD) on musculoskeletal injury and healing remain largely unknown.

HYPOTHESIS/PURPOSE: The purpose of this study was to evaluate the biomechanical effects of CBD and THC on tendon-to-tendon healing in a rat Achilles tendon repair model. The hypothesis was that rats administered CBD would demonstrate decreased tensile load to failure of surgically repaired Achilles tendons compared with the THC and control groups.

STUDY DESIGN

Controlled laboratory study.

METHODS

A total of 33 Sprague Dawley rats underwent Achilles tendon surgical transection and repair and were randomized to receive subcutaneous injection of THC, CBD, or vehicle once daily starting on the day of surgery and for 5 total days. After sacrifice, biomechanical tensile load-displacement testing was performed to determine Achilles tendon load to failure and stiffness. Data were analyzed by 1-way analysis of variance.

RESULTS

The THC group demonstrated the highest median load to failure, 18.7 N (95% CI, 15.3-19.2 N); the CBD group had the second highest at 16.9 N (95% CI, 15.1-19.8 N), and the control group had the lowest at 14.4 N (95% CI, 12.1-18.3 N). Stiffness was highest in the THC group at 4.1 N/mm (95% CI, 2.7-5.1 N/mm) compared with 3.6 N/mm (95% CI, 2.9-4.1 N/mm) for the CBD group and 3.6 N/mm (95% CI, 2.8-4.3 N/mm) for the control group. No statistically significant differences for strength and stiffness were observed between the groups.

CONCLUSION

In this pilot study using an animal tendon-to-tendon repair model, neither THC nor CBD resulted in altered biomechanical characteristics compared to control.

CLINICAL RELEVANCE

Cannabinoids do not appear to adversely affect Achilles tendon healing.

摘要

背景

大麻在运动员和美国人群中很常见。大麻素目前正在被评估为治疗慢性疼痛的阿片类药物替代品。然而，娱乐性和/或医疗使用 delta-9-四氢大麻酚（THC）和大麻二酚（CBD）对肌肉骨骼损伤和愈合的影响在很大程度上仍不清楚。

假设/目的：本研究旨在评估 CBD 和 THC 对大鼠跟腱修复模型中肌腱对肌腱愈合的生物力学影响。假设是接受 CBD 治疗的大鼠与 THC 和对照组相比，手术修复的跟腱的拉伸破坏负荷会降低。

研究设计

对照实验室研究。

方法

共有 33 只 Sprague Dawley 大鼠接受跟腱手术横断和修复，并随机接受每日一次的皮下注射 THC、CBD 或载体，从手术当天开始连续 5 天。处死动物后，进行生物力学拉伸负荷-位移测试，以确定跟腱的破坏负荷和刚度。数据采用单因素方差分析进行分析。

结果

THC 组的破坏负荷中位数最高，为 18.7 N（95%CI，15.3-19.2 N）；CBD 组次之，为 16.9 N（95%CI，15.1-19.8 N）；对照组最低，为 14.4 N（95%CI，12.1-18.3 N）。THC 组的刚度最高，为 4.1 N/mm（95%CI，2.7-5.1 N/mm），CBD 组为 3.6 N/mm（95%CI，2.9-4.1 N/mm），对照组为 3.6 N/mm（95%CI，2.8-4.3 N/mm）。组间强度和刚度无统计学差异。