National Reference Laboratory of Veterinary Drug Residues (HZAU) and MAO Key Laboratory for Detection of Veterinary Drug Residues, Huazhong Agricultural University, Wuhan, Hubei, China.
Department of Chemistry, College of Science, Huazhong Agricultural University, Wuhan, China.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2021 Aug;38(8):1364-1375. doi: 10.1080/19440049.2021.1916096. Epub 2021 Jun 7.
Sulphonamides (SAs) are widely used in animal husbandry. In our work, based on multi-walled carbon nanotubes, a novel residue method was developed for highly sensitive and determination trace levels of sulfamethoxazole, acetyl sulfamethoxazole and aditoprim in edible swine tissues by LC-MS/MS with magnetic solid-phase extraction. The samples were extracted using 2% ammoniated acetonitrile and purified by magnetic solid phase extraction (MSPE). Under the optimal conditions, good linearity was obtained ranging from 5 to 160 μg kg. The limits of detection (LOD) and quantification (LOQ) were 2 μg kg and 5 μg kg respectively. The average recoveries were 73.9-94.8% at different spiking levels. The inter-day RSDs were 6.2-10.7% and the intra-day RSDs were 2.4-5.4%. MSPE based on multi-walled carbon nanotubes was a simple and efficient method to enrich and separate the analyses and could be successfully applied for extraction of sulfamethoxazole, acetyl sulfamethoxazole and aditoprim residues in swine tissues.
磺胺类药物（SAs）在畜牧业中被广泛应用。在我们的工作中，基于多壁碳纳米管，我们开发了一种新的残留方法，用于通过 LC-MS/MS 结合磁固相萃取法，对食用猪组织中的磺胺甲恶唑、乙酰磺胺甲恶唑和氨苯砜进行痕量水平的高灵敏度和测定。样品用 2%氨化乙腈提取，并用磁固相萃取（MSPE）进行纯化。在最佳条件下，从 5 到 160μg kg 之间得到了良好的线性关系。检测限（LOD）和定量限（LOQ）分别为 2μg kg 和 5μg kg。在不同添加水平下，平均回收率为 73.9-94.8%。日间 RSD 为 6.2-10.7%，日内 RSD 为 2.4-5.4%。基于多壁碳纳米管的 MSPE 是一种简单有效的富集和分离分析的方法，可以成功地应用于猪组织中磺胺甲恶唑、乙酰磺胺甲恶唑和氨苯砜残留的提取。
