Elastocapillary effect in self-repair of proboscises of butterflies and moths.

HYPOTHESIS

Self-repair in living organisms, without tissue regeneration or regrowth, is rare. Recent discovery that butterflies can self-repair the proboscis after the two halves (galeae) have been separated raised a question about the physical mechanism allowing them to reunite the parts. We discovered that butterflies pump saliva during repair of their proboscises. We then hypothesized that saliva spreading along the food canal of the proboscis would create capillary forces capable of bringing the galeae together.

EXPERIMENT

To test the hypothesis, we distinguished capillary forces from muscular action of the galeae by sedating butterflies and video tracking retraction of the saliva menisci during galeal separation. To theoretically show capillary adhesion, the elastic moduli of the galeae were measured, and the galeal profiles were extracted from videos as a function of time. The values were then fitted with a mathematical model based on an augmented Euler-Bernoulli beam theory whereby each galea was treated as a beam bent by capillary forces due to saliva. We also evaluated friction forces that prevented disjoining of the galea at the tip of their separation.

FINDINGS

The results showed that butterflies use saliva to repair their proboscises via capillary adhesion, and theoretically supported the role of saliva in providing the necessary capillary forces to bring the galeae together. Tangential shear forces acting parallel to the galea at the tip of their separation are caused primarily by friction between the cuticular linking structures.