Krantz S, Lober M, Thiele M, Teuscher E
Institute of Biochemistry, Ernst Moritz Arndt University, Greifswald/GDR.
Exp Clin Endocrinol. 1988 May;91(2):155-60. doi: 10.1055/s-0029-1210738.
Adhesion of bovine endothelial cells on fibronectin and collagen before and after nonenzymatic glycation in vitro has been studied. Nonenzymatic glycation of these proteins reduced their ability to bind endothelial cells. Furthermore, nonenzymatically glycated fibronectin failed to bind to normal and nonenzymatically glycated gelatin and to fibrin. So gelatin and fibrin Sepharoses can be used to separate highly glycated fibronectins from fibronectins with a low degree of nonenzymatic glucose substitution. Sodium dodecylsulfate polyacrylamide gel electrophoresis did not demonstrate a covalent cross-link between nonenzymatically glycated fibronectins. These results present further evidences for the role of nonenzymatic glycation of proteins in the development of vascular complications in long-term diabetes and of atherosclerosis.
研究了体外非酶糖基化前后牛内皮细胞在纤连蛋白和胶原蛋白上的黏附情况。这些蛋白质的非酶糖基化降低了它们结合内皮细胞的能力。此外，非酶糖基化的纤连蛋白无法与正常及非酶糖基化的明胶和纤维蛋白结合。因此，明胶和纤维蛋白琼脂糖可用于从非酶葡萄糖取代程度低的纤连蛋白中分离高度糖基化的纤连蛋白。十二烷基硫酸钠聚丙烯酰胺凝胶电泳未显示非酶糖基化纤连蛋白之间存在共价交联。这些结果为蛋白质非酶糖基化在长期糖尿病血管并发症和动脉粥样硬化发展中的作用提供了进一步证据。
