Delaby Constance, Vialaret Jérôme, Hirtz Christophe, Lefebvre Thibaud, Herkert Matthias, Puy Hervé, Lasocki Sigismond, Lehmann Sylvain
INM, Université de Montpellier, INSERM, CHU Montpellier, IRMB, Laboratoire de Biochimie Protéomique, Montpellier, France.
Department of Neurology, Sant Pau Memory Unit, Institut d'Investigacions Biomèdiques Sant Pau - Hospital de Sant Pau, Universitat Autònoma de Barcelona, Barcelona, Spain.
Bioanalysis. 2021 Jul;13(13):1029-1035. doi: 10.4155/bio-2021-0069. Epub 2021 Jun 8.
To compare methods of quantifying serum hepcidin (based on MS and ELISA) and their ability to diagnose true iron deficiency anemia in critically ill patients. Serum hepcidin was measured in 119 critically ill patients included in the HEPCIDANE clinical trial, using either an ultra-sensitive ELISA kit (from DRG) or two different MS methods. The results show a good correlation between the different methods studied. The Bland-Altman analysis and the Kappa test for clinical groups show a good or very good agreement between the different tests. ELISA or MS show a satisfactory commutability to quantify serum hepcidin. This is of great importance for the determination of therapeutic strategies in iron deficiency.
比较定量血清铁调素的方法（基于质谱和酶联免疫吸附测定法）及其诊断危重症患者真性缺铁性贫血的能力。在纳入HEPCIDANE临床试验的119例危重症患者中，使用超灵敏酶联免疫吸附测定试剂盒（来自DRG）或两种不同的质谱方法测量血清铁调素。结果显示，所研究的不同方法之间具有良好的相关性。临床组的布兰德-奥特曼分析和kappa检验表明，不同检测方法之间具有良好或非常好的一致性。酶联免疫吸附测定法或质谱法在定量血清铁调素方面显示出令人满意的互换性。这对于缺铁性疾病治疗策略的确定非常重要。
