College of Basic Medicine, Qingdao University, Qingdao, Shandong, China.
Food Funct. 2021 Jun 8;12(11):4960-4971. doi: 10.1039/d0fo03113e.
The composition and structure of gut microbiota plays an important role in obesity induced by a high-fat diet (HFD) and related metabolic syndrome (MetS). Previous studies have shown that galacto-oligosaccharides (GOSs) have an effective anti-obesity effect. In this study, we aimed to investigate the effect of enzymatically synthesized α-galacto-oligosaccharides (ES-α-GOSs) on MetS and gut microbiota dysbiosis in HFD-fed mice, and to further investigate whether the attenuation of MetS is associated with the modulation of gut microbiota. Our results indicated that ES-α-GOS could notably ameliorate obesity-related MetS, including hyperlipidemia, insulin resistance and mild inflammation. The subsequent analysis of gut microbiota further showed that ES-α-GOS supplements can significantly modulate the overall composition of the gut microbiota and reverse the gut microbiota disorder caused by HFD feeding. Moreover, Spearman correlation analysis showed that 40 key bacteria reversed by ES-α-GOS were highly associated with metabolic parameters. These results suggested that ES-α-GOSs could serve as a potential candidate for preventing obesity-induced MetS in association with the modulation of gut microbiota.
肠道微生物群落的组成和结构在高脂肪饮食（HFD）诱导的肥胖和相关代谢综合征（MetS）中起着重要作用。先前的研究表明，半乳糖低聚糖（GOS）具有有效的抗肥胖作用。在这项研究中，我们旨在研究酶法合成的α-半乳糖低聚糖（ES-α-GOS）对 HFD 喂养小鼠 MetS 和肠道微生物群落失调的影响，并进一步研究 MetS 的衰减是否与肠道微生物群落的调节有关。我们的结果表明，ES-α-GOS 可显著改善肥胖相关的 MetS，包括高血脂、胰岛素抵抗和轻度炎症。随后对肠道微生物群落的分析进一步表明，ES-α-GOS 补充剂可显著调节肠道微生物群落的整体组成，并逆转 HFD 喂养引起的肠道微生物群落失调。此外，Spearman 相关分析显示，ES-α-GOS 逆转的 40 种关键细菌与代谢参数高度相关。这些结果表明，ES-α-GOS 可作为一种预防肥胖相关 MetS 的潜在候选物，与肠道微生物群落的调节有关。
