通过向兔网织红细胞裂解物中添加L-脯氨酸-5-羧酸诱导真核翻译起始因子4E（eIF-4E）磷酸化。

Induction of eIF-4E phosphorylation by the addition of L-pyrroline-5-carboxylic acid to rabbit reticulocyte lysate.

作者信息

Mick S J, Abramson R D, Ray B K, Merrick W C, Thach R E, Hagedorn C H

机构信息

Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110.

出版信息

FEBS Lett. 1988 Aug 29;236(2):484-8. doi: 10.1016/0014-5793(88)80082-6.

DOI:10.1016/0014-5793(88)80082-6

PMID:3410057

Abstract

Addition of L-pyrroline-5-carboxylic acid to reticulocyte lysates inhibits protein synthesis and induced phosphoproteins of 25 and 14 kDa. The 25 kDa phosphoprotein had the same Mr and pI as phosphorylated eIF-4E. Incubation of lysates with L-pyrroline-5-carboxylic acid did not alter the crosslinking of eIF-4E to reovirus mRNA caps. These results suggest that modifications of the translational apparatus other than eIF-4E phosphorylation may mediate the inhibitory effect seen with L-pyrroline-5-carboxylic acid and/or that phosphorylation of eIF-4E may effect functions subsequent to its interaction with the mRNA cap such as protein-protein interactions with other cap-specific translation factors.

摘要

向网织红细胞裂解物中添加L-脯氨酸-5-羧酸会抑制蛋白质合成，并诱导产生25 kDa和14 kDa的磷酸化蛋白。25 kDa的磷酸化蛋白与磷酸化的真核起始因子4E（eIF-4E）具有相同的相对分子质量（Mr）和等电点（pI）。将裂解物与L-脯氨酸-5-羧酸一起孵育不会改变eIF-4E与呼肠孤病毒mRNA帽的交联。这些结果表明，除了eIF-4E磷酸化之外，翻译装置的修饰可能介导了L-脯氨酸-5-羧酸所产生的抑制作用，和/或eIF-4E的磷酸化可能会影响其与mRNA帽相互作用之后的功能，例如与其他帽特异性翻译因子的蛋白质-蛋白质相互作用。