Daegu University, Gyeongsan-Si, Gyeongsangbuk-do, Republic of Korea.
Chembiochem. 2021 Nov 3;22(21):3010-3026. doi: 10.1002/cbic.202100219. Epub 2021 Jun 22.
The attachment of a single O-linked β-N-acetylglucosamine (O-GlcNAc) to serine and threonine residues of numerous proteins in the nucleus, cytoplasm, and mitochondria is a reversible post-translational modification (PTM) and plays an important role as a regulator of various cellular processes in both healthy and disease states. Advances in strategies and tools that allow for the detection of dynamic O-GlcNAcylation on cellular proteins have helped to enhance our initial and ongoing understanding of its dynamic effects on cellular stimuli and given insights into its link to the pathogenesis of several chronic diseases. Furthermore, chemical genetic strategies and related tools have been successfully applied to a myriad of biological systems with a new level of spatiotemporal and molecular precision. These strategies have started to be used in studying and controlling O-GlcNAcylation both in vivo and in vitro. In this minireview, overviews of recent advances in molecular tools being applied to the detection and identification of O-GlcNAcylation on cellular proteins as well as on individual proteins are provided. In addition, chemical genetic strategies that have already been applied or are potentially usable in O-GlcNAc functional are also discussed.
蛋白质的细胞核、细胞质和线粒体中，许多蛋白质的丝氨酸和苏氨酸残基上连接单个 O-连接的β-N-乙酰葡萄糖胺（O-GlcNAc）是一种可逆的翻译后修饰（PTM），作为健康和疾病状态下各种细胞过程的调节剂，发挥着重要作用。用于检测细胞蛋白上动态 O-GlcNAcylation 的策略和工具的进步，有助于增强我们对其对细胞刺激的动态影响的初步和持续理解，并深入了解其与几种慢性疾病发病机制的联系。此外，化学遗传策略和相关工具已成功应用于具有新的时空和分子精度水平的无数生物系统。这些策略已开始用于研究和控制体内和体外的 O-GlcNAcylation。在这篇简评中，提供了应用于检测和鉴定细胞蛋白和个别蛋白质上 O-GlcNAcylation 的分子工具的最新进展概述。此外，还讨论了已经或可能在 O-GlcNAc 功能中应用的化学遗传策略。
