Lu C C, Wei R X, Deng D H, Luo Z Y, Abdulai M, Liu H H, Kang B, Hu S Q, Li L, Xu H Y, Hu J W, Wei S H, Han C C
Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan, 611130, P.R. China.
Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan, 611130, P.R. China.
Poult Sci. 2021 Jul;100(7):101208. doi: 10.1016/j.psj.2021.101208. Epub 2021 Apr 21.
To explored the difference of goose fatty liver formation induced-by different types of sugar from the intestinal physiology and the gut microflora, an integrated analysis of intestinal physiology and gut microbiota metagenomes was performed using samples collected from the geese including the normal-feeding geese and the overfed geese which were overfed with maize flour or overfeeding dietary supplementation with 10% sugar (glucose, fructose or sucrose, respectively), respectively. The results showed that the foie gras weight of the fructose group and the sucrose group was heavier (P < 0.05) than other groups. Compared with the control group, the ileum weight was significantly higher (P < 0.01), and the cecum weight was significantly lower in the sugar treatment groups (P < 0.001). Compared with the control group, the ratio of villi height to crypt depth in the fructose group was the highest in jejunum (P < 0.05); the trypsin activity of the ileum was higher in the fructose group and the sucrose group (P < 0.05). At the phylum level, Firmicutes, Proteobacteria and Bacteroidetes were the main intestinal flora of geese; and the abundance of Firmicutes in the jejunum was higher in the sugar treatment groups than that of the maize flour group. At the genus level, the abundance of Lactobacillus in the jejunum was higher (P < 0.05) in the sugar treatment groups than that of the maize flour group. In conclusion, forced-feeding diet supplementation with sugar induced stronger digestion and absorption capacity, increased the abundance of Firmicutes and Bacteroidetes and the abundance of Lactobacillus (especially fructose and sucrose) in the gut. So, the fructose and sucrose had higher induction on hepatic steatosis in goose fatty liver formation.
为了从肠道生理学和肠道微生物群方面探究不同类型糖类诱导鹅脂肪肝形成的差异，本研究使用从正常饲养鹅以及分别用玉米粉过量饲喂或用10%糖类（分别为葡萄糖、果糖或蔗糖）进行过量饮食补充的过量饲喂鹅中采集的样本，对肠道生理学和肠道微生物群宏基因组进行了综合分析。结果表明，果糖组和蔗糖组的鹅肥肝重量比其他组更重（P<0.05）。与对照组相比，糖类处理组的回肠重量显著更高（P<0.01），盲肠重量显著更低（P<0.001）。与对照组相比，果糖组空肠绒毛高度与隐窝深度的比值最高（P<0.05）；果糖组和蔗糖组回肠的胰蛋白酶活性更高（P<0.05）。在门水平上，厚壁菌门、变形菌门和拟杆菌门是鹅的主要肠道菌群；糖类处理组空肠中厚壁菌门的丰度高于玉米粉组。在属水平上，糖类处理组空肠中乳酸杆菌的丰度高于玉米粉组（P<0.05）。总之，强制饲喂添加糖类的日粮诱导了更强的消化吸收能力，增加了肠道中厚壁菌门、拟杆菌门以及乳酸杆菌（尤其是果糖和蔗糖组）的丰度。因此，果糖和蔗糖在鹅脂肪肝形成中对肝脂肪变性的诱导作用更强。
