高脂肪/高碳水化合物膳食对慢性脊髓损伤成人白细胞群体的影响。
The effects of a high-fat/high-carbohydrate meal on leukocyte populations in adults with chronic spinal cord injury.
机构信息
School of Health & Exercise Sciences, University of British Columbia, Kelowna, Canada.
International Collaboration on Repair Discoveries (ICORD), Blusson Spinal Cord Centre (BSSC), University of British Columbia, Vancouver, Canada.
出版信息
Spinal Cord Ser Cases. 2021 Jun 8;7(1):49. doi: 10.1038/s41394-021-00412-7.
STUDY DESIGN
Secondary analysis of aggregated case series data.
OBJECTIVES
To examine the effects of a high-fat/high-carbohydrate meal on leukocyte populations in adults with a chronic SCI.
SETTING
University-based laboratories in British Columbia, Canada.
METHODS
Ten individuals (M = 9) with a traumatic SCI (>1-year post-injury; M = 15.5 years; n = 2 sensory complete, n = 7 motor complete) participated in this study. Participants arrived fasted (≥12 h) prior to both the control (quiet sitting, no food/drink) and experimental meal conditions (high-fat/high-carb meal: 880 kcal, 52 g fat, 73 g carbohydrates, 29 g protein). Blood samples were taken in the fasted state and at 120-min post-meal/baseline in both conditions. Immune cell counts were assessed using multi-color flow cytometry.
RESULTS
A significant time × condition interaction effect was seen in CD3+, CD4+, and CD8+ T cells as well as CD56+ and CD3+/CD56+ natural killer (NK) cells (p < 0.05). CD14+/CD16+ monocytes and CD19+ B cells approached a significant time × condition interaction (p < 0.07). A main effect of time was observed in CD19+ B cells (p < 0.05). Cell counts for T-lymphocytes and NK cells followed the general trend of an increase in the control condition from baseline to 120-min with no change observed following the experimental meal condition.
CONCLUSIONS
Following the HFHC meal, immune cells did not show the same general increase observed following the control condition. Future research is needed to determine if there are any potential consequences of these immune cell responses in immunosuppressed populations and if other factors (e.g., diurnal variation) might influence immune cell response.
研究设计
聚合病例系列数据的二次分析。
目的
检查高脂肪/高碳水化合物膳食对慢性 SCI 成人白细胞群体的影响。
设置
加拿大不列颠哥伦比亚大学的实验室。
方法
10 名（M=9）创伤性 SCI 患者（损伤后>1 年；M=15.5 岁；n=2 感觉完全性，n=7 运动完全性）参与了这项研究。参与者在禁食（≥12 小时）后到达，分别接受对照（安静坐姿，无食物/饮料）和实验餐条件（高脂肪/高碳水化合物餐：880 卡路里，52 克脂肪，73 克碳水化合物，29 克蛋白质）。在禁食状态和餐后 120 分钟/基线时，在两种情况下采集血液样本。使用多色流式细胞术评估免疫细胞计数。
结果
在 CD3+、CD4+和 CD8+T 细胞以及 CD56+和 CD3+/CD56+自然杀伤（NK）细胞中观察到时间与条件的显著交互作用（p<0.05）。CD14+/CD16+单核细胞和 CD19+B 细胞接近时间与条件的显著交互作用（p<0.07）。在 CD19+B 细胞中观察到时间的主要效应（p<0.05）。T 淋巴细胞和 NK 细胞的细胞计数遵循对照条件从基线到 120 分钟增加的一般趋势，在实验餐后条件下没有观察到变化。
结论
在 HFHC 餐后，免疫细胞没有表现出与对照条件相同的普遍增加。需要进一步研究以确定这些免疫细胞反应在免疫抑制人群中是否存在任何潜在后果，以及其他因素（例如，昼夜变化）是否会影响免疫细胞反应。
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