Steffenrud S, Salari H
Department of Medicine, University of British Columbia, Vancouver General Hospital, Canada.
J Chromatogr. 1988 May 13;427(1):1-7. doi: 10.1016/0378-4347(88)80099-9.
A novel mobile phase containing heptafluorobutyric acid has been used for ion-interaction high-performance liquid chromatography of leukotrienes, lipoxins and related compounds on octadecylsilane silica columns. The use of a hydrophobic perfluorinated carboxylic acid as an ion-interaction agent at optimized concentration and pH permitted rapid, isocratic separation of leukotrienes, lipoxins and monohydroxyeicosatetraenoic acids. A completely volatile mobile phase suitable for preparative chromatography was obtained by using triethylamine as the base for pH adjustment. With this novel mobile phase, leukotrienes and monohydroxyeicosatetraenoic acids were completely separated in less than 15 min.
一种含有七氟丁酸的新型流动相已用于在十八烷基硅烷硅胶柱上对白三烯、脂氧素及相关化合物进行离子相互作用高效液相色谱分析。在优化的浓度和pH条件下，使用疏水性全氟羧酸作为离子相互作用剂，可以对白三烯、脂氧素和单羟基二十碳四烯酸进行快速等度分离。通过使用三乙胺作为调节pH的碱，获得了适用于制备色谱的完全挥发性流动相。使用这种新型流动相，白三烯和单羟基二十碳四烯酸在不到15分钟的时间内就完全分离了。
