National Engineering Research Center for Wheat, Henan Agricultural University, #15 Longzihu College District, Zhengzhou, 450046, Henan Province, People's Republic of China.
National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, #15 Longzihu College District, Zhengzhou, 450046, Henan Province, People's Republic of China.
Bull Environ Contam Toxicol. 2021 Aug;107(2):320-326. doi: 10.1007/s00128-021-03283-8. Epub 2021 Jun 10.
Cadmium (Cd), a toxic heavy metal, is harmful to plants and human health. Glutathione (GSH) could alleviate Cd toxicity of plant species, whereas its mechanism responsible for wheat remains poorly understood. Here, we found that exogenous GSH application significantly increased the fresh and dry weight, root elongation, chlorophyll contents, while decreased the contents of malondialdehyde (MDA) and GSH, and translocation factor of Cd compared with Cd treatment. Moreover, GSH application significantly increased activities of antioxidant enzymes and expression of related genes, which involved in GSH synthesis, especially in roots. In addition, we found that GSH application suppressed Cd-induced expression of metal transporter genes TaNramp1, TaNramp5, TaHMA2, TaHMA3, TaLCT1 and TaIRT2 in roots. Taken together, our results suggested that GSH could alleviate Cd toxicity in wheat by increasing GSH synthesis gene expression or suppressing Cd transporter genes expression, and further affecting Cd uptake and translocation in wheat plants.
镉（Cd）是一种有毒重金属，对植物和人类健康有害。谷胱甘肽（GSH）可以减轻植物物种的 Cd 毒性，但其对小麦的作用机制仍知之甚少。在这里，我们发现与 Cd 处理相比，外源 GSH 的应用显著增加了鲜重和干重、根伸长、叶绿素含量，而降低了丙二醛（MDA）和 GSH 的含量以及 Cd 的迁移系数。此外，GSH 的应用显著增加了抗氧化酶的活性和相关基因的表达，这些基因参与 GSH 的合成，尤其是在根中。此外，我们发现 GSH 的应用抑制了 Cd 诱导的金属转运蛋白基因 TaNramp1、TaNramp5、TaHMA2、TaHMA3、TaLCT1 和 TaIRT2 在根中的表达。总之，我们的结果表明，GSH 通过增加 GSH 合成基因的表达或抑制 Cd 转运蛋白基因的表达来减轻小麦中的 Cd 毒性，并进一步影响小麦植株对 Cd 的吸收和转运。
